甘蔗花叶病毒南城分离物外壳蛋白基因序列测定及分析  

Sequencing and Analysis of Coat Protein Gene of Sugarcane Mosaic Virus-NCH Isolate

作　　者：蒋军喜[1] 钱亚娟[2] 谢艳[2] 阙海勇[1] 罗有强[1] 向妙莲[1] 

机构地区：[1]江西农业大学农学院,江西南昌330045 [2]浙江大学生物技术研究所,浙江杭州310029

出　　处：《江西农业大学学报》2008年第3期464-467,共4页Acta Agriculturae Universitatis Jiangxiensis

基　　金：江西省自然科学基金资助项目(0330077)

摘　　要：从江西南城甘蔗病株中获得1个甘蔗花叶病毒（Sugarcane mosaic virus,SCMV）分离物,命名为SCMV-NCH。根据SCMV外壳蛋白（CP）基因N-端和C-端保守序列设计引物,对SCMV-NCH的CP基因进行RT-PCR扩增、克隆和测序,获得924个核苷酸（nt）,推断编码308个氨基酸（aa）的近全长CP基因序列。与SCMV亚组SCMV、高粱花叶病毒（SrMV）、玉米矮花叶病毒（MDMV）、约翰逊草花叶病毒（JGMV）、玉米花叶病毒（ZeMV）、白草花叶病毒（PenMV）等6种病毒代表性株系外壳蛋白氨基酸序列同源性比较结果显示,SCMV-NCH与SCMV的同源性最高（86.2%）,与其他5种病毒同源性相对较低（56.4%～72.2%）。与SCMV13个分离物外壳蛋白氨基酸序列进行同源性比较并构建关系树,结果显示SCMV-NCH与我国浙江2个分离物（Yuhang和Xiaosh）具有最近的亲缘发生关系。A isolate of Sugarcane mosaic virus（SCMV） named SCMV-NCH was obtained from infected sugarcane in Nancheng County,Jiangxi Province.A primer pair of SCMV targeted to the N-terminal and C-terminal of coat protein（CP） gene was designed and used to amplify the CP gene of SCMV-NCH by using RT-PCR method.The PCR product was cloned and sequenced.It had 924 nucleotides（nt）,encoding 308 amino acids （aa）.The nearly full-length CP sequence of 308 aa of SCMV-NCH was compared with the counterpart of SCMV,Sorghum mosaic virus（SrMV）,Maize dwarf mosaic virus （MDMV）;Johnsongrass mosaic virus（JGMV）,Zea mosaic virus（ZeMV） and Pennisetum mosaic virus（PenMV）,the six virus species in SCMV subgroup in Potyvirus,respectively.A high level of sequence homology（86.2%） was observed between SCMV-NCH and SCMV-A,while only 56.4% to 72.2% sequence homology was found between SCMV-NCH and the other five virus species.The 308 aa sequence of SCMV-NCH was then compared with the counterpart of 13 selected strains（isolates） of SCMV,and a phylogenetic tree was constructed.It showed that SCMV-NCH was closely related to SCMV-Yuhang and SCMV-Xiaosh,the two sugarcane isolates in Zhejiang Province,China.

关 键 词：甘蔗花叶病毒 外壳蛋白基因 序列测定 亲缘关系分析 

分 类 号：S432.41[农业科学—植物病理学] S566.1[农业科学—农业昆虫与害虫防治]