马尾松毛虫多功能氧化酶测定正交试验优化研究  

Optimization of Test Conditions for Mixed-function Oxidases(MFO) in Dendrolimus punctatus Walker by Orthogonal Experiment

作　　者：庞会忠[1] 彭龙慧[1] 张金波[1] 杨斌[1] 唐春发[1] 汪辉武[1] 张玮[1] 

机构地区：[1]江西农业大学,江西南昌330045

出　　处：《江西农业大学学报》2008年第3期476-479,共4页Acta Agriculturae Universitatis Jiangxiensis

基　　金：江西省自然科学基金项目(赣科基办字[2005]3号)

摘　　要：应用L16(43)正交试验设计对影响马尾松毛虫多功能氧化酶细胞色素P450测定的因素PMSF浓度、酶原稀释比和离心速度进行优化研究。结果表明供试的16组复配组合中,组合A1B1D1(选用0.2 mol/L PMSF浓度、酶原稀释比为2∶1,离心速度1为5 000 r/min)最优,按照该组合的条件进行马尾松毛虫MFO酶细胞色素P450测定,效果最佳。Using the orthogonal table L16（4^3）,the concentration of PMSF,the dilution ratio of mixed-function oxidases（MFO） and centrifugation velocity,3 factors which affecting the test of MFO in Dendrolimus punctatus were optimized by orthogonal experiment.The results showed that the combination A1B1D1 [0.2mol/L PMSF,dilution ratio of MFO 2∶1,centrifugation velocity1：5 000 turn/min] was the best one in all of the 16 combinations and using combination A1B1D1,the effect of cytochrome P450 of MFO testing in Dendrolimus punctatus Walker was the best in the orthogonal experiment.

关 键 词：正交试验 马尾松毛虫 多功能氧化酶 细胞色素P450 

分 类 号：Q946.5[生物学—植物学]