血清锰的石墨炉原子吸收分光光度计测定方法  

Determination of serum manganese with graphite AAS

作　　者：王先良[1] 王小利[2] 张金良[1] 杨永坚[3] 赵秀阁[1] 全占军[1] 钱岩[1] 王红梅[1] 

机构地区：[1]中国环境科学研究院环境污染与健康研究室,北京100012 [2]北京工业大学生命科学学院,北京100022 [3]安徽医科大学公共卫生学院,合肥230032

出　　处：《微量元素与健康研究》2008年第3期48-49,64,共3页Studies of Trace Elements and Health

摘　　要：目的:建立一种血清锰的石墨炉原子吸收分光光度检测方法。方法:将血清和0.44 M的HNO3按1∶9充分稀释后,15000 r/m高速离心10 min,取上清20μl直接测定。结果:以PE-AA800型原子吸收分光光度计为对象,发现最佳灰化温度为1300℃,最佳原子化温度为1900℃,最佳净化温度为2450℃和基底改进剂为5μg钯+3μg硝酸镁;平均回收率可达到97.75%;变异系数(CV)为5.0%;特征浓度为6.37610 pg/0.0044A,检出限为6.7 pg。结论:该法为一种可信度较高,操作性较强的血清锰的实用测定方法。Objective： To set up a new method of detecting the level of manganese in serum with the graphite atomic absorption spectroscopy（AAS）, Methods： The serum is diluted with HNO3 and centrifuged 10 minutes by 15000 r/m first and the solution above in the tube is going to be determined. Results： The optimal temperatures for cineration, atomization and clearance are 1300℃, 1900℃ and 2450℃ with the matrix modifier of a unit of 5μg Pd and 3μg Mg（ NO3 ）2, respectively, the average reclamation rate is 97.75 % and the coefficient of variation is about 5%; the sensitivity is 6. 37610 pg / 0. 0044A and the minimal level can be detected is 6.7 pg. Conclusions： this is a practicable and credible method for detection serum Mn with AAS.

关 键 词：AAS 血清 锰 

分 类 号：O657.31[理学—分析化学]