鱼腥草中挥发油的提取分析及其抗菌抗病毒作用的研究  

Extraction of Volatile Oil from Houttuynia Cordata and Its Anti-biotic and Anti-virus Activities

作　　者：张薇[1] 卢芳国[2] 潘双银[1] 李删[2] 

机构地区：[1]湖南农业大学,湖南长沙410128 [2]湖南中医药大学

出　　处：《实用预防医学》2008年第2期312-316,共5页Practical Preventive Medicine

基　　金：湖南省科技厅资助项目(2007FJ4144);湖南省自然基金资助项目(06JJ2055)

摘　　要：目的研究鱼腥草挥发油的提取方法及挥发油的抗菌抗病毒作用。方法以新鲜野生紫色茎鱼腥草为原料,采用水蒸气蒸馏法提取挥发油,利用气相色谱与质谱联用(GC-MS)分析检测了挥发油的化学成分及含量,运用琼脂扩散法分析检测鱼腥草挥发油的抗菌作用,并用鸡胚培养法和微量血凝试验测定了鱼腥草挥发油对流感病毒增殖的抑制作用。结果与结论水蒸气蒸馏法提取鱼腥草挥发油的最佳条件是将鱼腥草捣碎研磨后,于100℃水浴中,水蒸气蒸馏提取4 h;从提取物中鉴定出了癸酰乙醛、2-十一烷酮等26种化学组分;所提取的鱼腥草挥发油对乙型溶血性链球菌、金黄色葡萄球菌、绿脓假单胞菌、大肠埃希菌均有一定的抑制作用,其中对金黄色葡萄球菌抑制作用最大;鱼腥草挥发油对流感病毒有抑制作用,药物质量浓度在31.25mg/ml时即能达到抑制病毒的增殖,浓度在250mg/ml时对鸡胚内流感病毒有4倍的抑制作用。Objective To explore the extraction technology of volatile oil from Houttuynia Cordata, and to observe the antibiotic and anti - virus activities of volatile oil. Methods Volatile oil in fresh wild caudex Houttuynia Cordata thunb was extracted by steam distillation. The chemical components in the extracted oil were identified and determined via GO - MS analysis. The antibiotic activity of the extract was measured by agar - diffusion assay, and the inhibitory effect on influenza virus was determined by chick embryo culture and blood coagulation test. Results＆Conclusions The best method for the extraction of the volatile oil from Houttuynia Cordata thunb was first to smash and grind the plant material and then to distill with steam for 4 h in a water bath of 100 ℃. Houttuyfonate, 2 - Undecanone and others altogether 2.5 compounds were,identified. Assay for antibiotic activity showed that the extract had inhibitory effect on β- hemolytic streptococcus, S. aureus, P. aeruginosa and E. coil, and that the highest inhibitory effect was on S. aureus. A higher than 31.2.5 mg/ml concentration of the extracted oil showed inhibitory effect on multiplication of influenza virus and a complete inhibition was obtained when the oil concentration reached 250mg/ml. The inhibitory effect on the virus was 4 times higher in the chicken embryo.

关 键 词：鱼腥草 挥发油 水蒸气蒸馏法 提取 GC-MS分析 抗菌、抗病毒作用 

分 类 号：R282[医药卫生—中药学]