免疫比浊法在梅毒螺旋体抗体定量检测中的临床应用与评价  

Clinical Application and Evaluation of Immune Turbidimetry in Treponema Pallidum Antibody Detection

作　　者：李学祥[1] 周善良 贾德强[1] 王慜杰[1] 齐军[1] 

机构地区：[1]中国医学科学院肿瘤医院,北京100021 [2]山东省临沂市肿瘤医院

出　　处：《实用预防医学》2008年第2期353-354,共2页Practical Preventive Medicine

摘　　要：目的评价免疫比浊法在梅毒螺旋体抗体检测中的临床应用价值。方法应用明胶颗粒凝集法(TPPA)和免疫比浊法(3TP)同时检测352份梅毒螺旋体抗体阴性样本和88份梅毒螺旋体抗体阳性样本。结果TPPA法和3TP法的灵敏度分别为100%、98.9%,特异性为100%、99.4%,两种方法的符合率为99.31%;对质控血清进行25次不连续测定,3TP法的测量值为(64.2±2.13)U,CV值为3.31%;3TP法检测值与TPPA法检测滴度之间显著相关,等级相关系数Rs=0.971,P<0.001。结论与TPPA法相比,3TP法的检测性能较好,具有较大的临床应用价值。Objective To evaluate the clinical values of immune turbidimetry method in the detection of treponema pallidum （TP） antibody. Methods A total of 352 TP antibody negative samples and 88 TP antibody positive samples were detected by using treponema pallidum particle agglutination （TPPA） assay and immune turbideimetry method （3TP） respectively. Results The sensitivity and the specificity of TPPA were both 100%, while the sensitivity and the specificity of 3TP were 98,9 % and 99.4 % respectively, The overall coincidence rate of the two methods was 99.31%. The value of the quality- control blood serum was about 64.2 ± 2.13U in 25 discontinuous detections and the coefficient of variation （OV） was 3.31%. The relationship between the values of 3TP and the titer of TPPA showed statistical significance, and the rank correlation coefficient （Rs） was 0. 971（ P〈 0. 001 ）. Conclusions The 3TP shows excellent performance in TP detection as compared with the TPPA, and 3TP is an appropriate method in diagnosis of syphilis.

关 键 词：梅毒螺旋体抗体 免疫比浊法 明胶颗粒凝集法 

分 类 号：R377.1[医药卫生—病原生物学]