HPV18 E7抗原HLA-DRB1*0301限制性Th表位的预测与鉴定  

Prediction and identification of HLA-DRB1*0301 restricted Th epitope derived from human papillomavirus 18 E7 antigen

作　　者：余剑琴[1] 郑飞云[1] 徐云升[2] 欧荣英[1] 张乾[1] 

机构地区：[1]温州医学院第一附属医院妇产科,浙江温州325000 [2]温州医学院第一附属医院皮肤科,浙江温州325000

出　　处：《温州医学院学报》2008年第3期193-197,共5页Journal of Wenzhou Medical College

基　　金：国家自然科学基金资助项目(30500537)

摘　　要：目的:初步鉴定人乳头瘤病毒18型E7抗原的HLA-DRB1*0301限制性辅助T淋巴细胞(T-HelperCell)表位。方法:用SYFPEITHI软件预测HPV18E7抗原HLA-DRB1*0301限制性Th表位,候选表位分别命名为P1、P2、P3,以固相多肽合成技术合成,并运用HPLC进行纯化和质谱法(MS)鉴定。以密度梯度法分离HLA-DRB1*0301阳性健康人外周血单核细胞(PBMC),合成肽刺激PBMC,用5-溴脱氧尿嘧啶核苷(Brdu)检测淋巴细胞增殖活性,流式细胞仪分析细胞表型。结果:多肽P1(HPV18E780-94)在体外刺激PBMC后能够有效诱导CD4+T淋巴细胞增殖。结论:P1(HPV18 E780-94)可能为HPV18E7抗原HLA-DRB1*0301限制性Th细胞表位。Objective：To identify BLA-DRB1^＊0301restricted Th epitope derived from human papillomavirus 18 E7 antigen. Methods： The primary structure of htraan papillomavirus 18 E7 antigen was analyzed using T cell epitope predictive algorithms SYFPEITHI and three epitope candidates （P1, P2 and P3） were obtained. The candidate epitopes were synthesized with solid phase strategies, purified with reverse phase HPLC and identified with mass spectrometry. Peptide-specific Th cells were induced from the peripheral blood mononuclear cells （PPMC） of HLA-DRB1^＊0301 positive healthy donors by stimulating candidate epitopes. Proliferative activity was analyzed with Brdu cell proliferation assay. Cell phenotype was analyzed by FACS. Results： Peptide P1 （HPV18 E780-94） could induce proliferative activity in vitro specifically and effectively. Conclusion： Peptide P1 （HPV18 E780-94） can be the HLA-DRB1^＊0301restricted Th epitope of human papillomavirus 18 E7 antigen.

关 键 词：人乳头瘤病毒 E7抗原 淋巴细胞 TH表位 

分 类 号：R392.11[医药卫生—免疫学]