环境激发吗啡条件性位置偏爱重现大鼠海马PSD-95的蛋白和DNA表达  

Expression of PSD-95 protein and DNA in Hippocampal of rat with morphine conditioned place preference after environment induced reinstatement

作　　者：朱华[1] 韩军鸽[1] 李兴彪[1] 吴淑珍[1] 陈鹏[2] 罗良鸣[3] 

机构地区：[1]温州医学院法医学系,浙江温州325035 [2]温州市公安局瓯海分局,浙江温州325000 [3]温州市公安局,浙江温州325000

出　　处：《温州医学院学报》2008年第3期233-236,共4页Journal of Wenzhou Medical College

基　　金：浙江省教育厅科研基金资助项目(20051166);温州市科技局科研基金资助项目(Y2005A039)

摘　　要：目的:检测吗啡条件性位置偏爱(conditioned place preference,CPP)重现大鼠海马区突触后致密质-95(PSD-95)的蛋白和DNA表达,观察PSD-95对吗啡成瘾记忆的影响。方法:建立大鼠吗啡CPP模型,自然消退后,通过环境来诱发CPP的重现,应用免疫组化和RT-PCR的方法,观察吗啡CPP重现组大鼠海马区PSD-95的表达,并与吗啡CPP消退组、生理盐水对照组进行比较。结果:吗啡CPP环境激发组、吗啡CPP消退组与生理盐水对照组相比,以及吗啡CPP环境激发组与吗啡CPP消退组相比,海马区PSD-95的表达明显降低,差异具有高度显著性(P<0.01)。结论:吗啡CPP重现和消退大鼠海马区PSD-95表达明显降低,海马区PSD-95可能未参与成瘾记忆。Objective： To observe the expression of protein and DNA of postsynaptic density protein95（PSD-95） in hippocampus of rats with morphine conditioned place preference（CPP） after environment induced reinstatement, and to evaluate the influence of PSD-95 on addiction memory. Methods： CPP rat model was established with morphine injection and reinstatement was induced by environment after extinction of CPP. The expression of PSD-95 protein and DNA in hippocampus of rats in reinstatement group of morphine CPP were observed, and was compared with those in extinction group of morphine CPP and control group. Results： The expression of PSD-95 in hippocampus was significantly decreased in rats in reinstatement and extinction group of morphine CPP compared with those in control group（P〈0.01）, and it was significantly decreased in reinstatement group compared with those in extinction group（P〈0.01）. Conclusion： The expression of PSD-95 in hippocampus is significantly decreased in rats in reinstatement and extinction group of morphine CPP. PSD-95 in hippocampus has take part or not in addict memory.

关 键 词：吗啡 条件性位置偏爱 环境激发 海马 突触后致密质-95 

分 类 号：R89[医药卫生—法医学] R36[医药卫生—临床医学]