壳聚糖固定化氨基酰化酶的研究  

Studies on Immobilization of Aminoacylase of Chitosan

作　　者：王伟[1] 杨开伦[1] 李华[2] 

机构地区：[1]新疆农业大学动物营养实验室,乌鲁木齐830052 [2]新疆农业大学化工学院,乌鲁木齐830052

出　　处：《新疆农业大学学报》2008年第3期21-24,共4页Journal of Xinjiang Agricultural University

基　　金：国家自然科学基金项目(30471262)

摘　　要：以壳聚糖为载体，戊二醛为交联剂，用吸附交联法对氨基酰化酶进行了固定化研究。结果表明，在pH值为6．0，温度30℃的条件下，0．1g壳聚糖微球与5mL 1％戊二醛交联后，固定0．8mg氨基酰化酶的固定化效果最佳。固定化酶的最适温度和pH值分别为50℃和7．0，而游离酶的最适温度为40℃，最适pH值为7．5，固定化酶在50～70℃都保持了较高的酶活力，热稳定性远高于游离酶，固定化酶的K。值为11．796×10 mol／L，较游离酶有所升高，该固定化酶具有良好的操作稳定性。In the experiment the aminoacylase was systematically studied by use of crosslinking method with chitosan as a vehicle,glutaraldehyde as crosslinking agent. The immobilized effect with 0.8 mg aminoacylase was the best, when the reaction temperature was 30 ℃,pH 6.0,0. 1 g of chitosan micropheres, 5 mL 1% solution of glularaldehyde. Optimal temperature and pH of the immobilized enzyme were 50 ℃ and 7.0 respectively,while the free enzyme was 40 ℃ and 7.5,respectively. The immobilized enzyme kept high activity between 50 ℃ and 70 ℃ and the temperature stability of the immobilized enzyme was better than free enzyme. The Km of the immobilized enzyme is 11. 796×10^-2 mol/L,which is higher than that of the free enzyme, and the immobilized enzyme also showed good operation stability.

关 键 词：氨基酰化酶 壳聚糖 固定化 

分 类 号：Q55[生物学—生物化学]