高效液相色谱法测定辛芍注射液中芍药苷含量  

Determination of Paeoniflorin in Xinshao Injection by HPLC

作　　者：马琳[1] 李勇军[1] 何迅[1] 兰燕宇[1] 王爱民[1] 王永林[1] 

机构地区：[1]贵阳医学院,贵州贵阳550004

出　　处：《中国药业》2008年第11期22-23,共2页China Pharmaceuticals

基　　金：贵州省中药现代化科技产业研究开发专项基金项目;项目编号:黔科合农字[2006]5003。

摘　　要：目的建立辛芍注射液的含量测定方法。方法采用Diamonsil C18柱(250mm×4.6mm,5μm),以甲醇-0.1%甲酸溶液(40∶60)为流动相,流速1.0mL/min,检测波长230nm,柱温40℃。结果芍药苷质量浓度的线性范围为49～173μg/mL,r=0.9999,平均回收率为98.44%,RSD=2.0%(n=6)。结论所用方法简单、准确、重现性好,适用于辛芍注射液的质量控制。Objective To establish a method for determining paeoniflorin in Xinshao Injection. Methods Diamonsil C18 column （250 mm×4.6mm,5 μm） was used with mixture of methanol-0. 1% formic acid （40：60） as the mobile phase and the flow rate of 1.0mL/min. The detection wavelength was 230 nm and the temperature of column was 40 ℃. Results The linear range of paeoniflorin was 49 - 173 μg/mL （r = 0. 999 9）. The average recovery rate was 98.44% and RSD was 2. 0% （n = 6）. Conclusion The method is simple,accurate,reproducible and suitable to be used in the quality control of Xinshao Injection.

关 键 词：辛芍注射液 赤芍 芍药苷 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学] R972