反相高效液相色谱法同时测定芩黄喉症胶囊3组分的含量  

Simultaneous Determination of Baicalin,Emodin and Chrysophanol in Qinhuanghouzheng Capsule by RP-HPLC

作　　者：徐迪[1] 霍理坚[1] 卓烨烨[1] 王定勇[2] 

机构地区：[1]华南农业大学制药工程系,广东广州510642 [2]广东药学院,广东广州510224

出　　处：《中国药业》2008年第11期28-29,共2页China Pharmaceuticals

摘　　要：目的建立同时测定芩黄喉症胶囊中黄芩苷、大黄素及大黄酚含量的反相高效液相色谱法。方法色谱柱为Agilent C18柱,流动相为甲醇-0.4%磷酸水溶液,梯度洗脱,流速为1.0mL/min,检测波长为254nm,柱温为室温。结果黄芩苷、大黄素及大黄酚的质量浓度分别在8.8～1389mg/L(r=0.9999),0.52～86.54mg/L(r=0.9996),0.42～62.42mg/L(r=0.9998)范围内与峰面积呈良好线性关系,平均回收率分别为99.8%,100.4%,99.8%;方法精密度及重现性良好,RSD均不大于1.3%。结论反相高效液相色谱法简便准确且效率高,可作为芩黄喉症胶囊中黄芩苷、大黄素及大黄酚含量的测定方法。Objective To establish a RP- HPLC method of the determination of baicalin, emodin, chrysophanol in Qinhuanghouzheng Capsule. Methods The column of Agilent C18 （150 mm×4. 6 mm, 5.0 μm） was used. The mobile phase composed of methanol-0. 4% phosphoric acid aqueous solution with gradient elution. The flow rate was 1.0 mL/min with UV detection wavelength at 254 nm. Results A good linearity was obtained with the correlation coefficients ranged from 8.8 - 1 389 mg/L（ r = 0. 999 9） ;0. 52 -86.54 mg/L （ r =0. 999 6）; 0.42- 62.42 mg/L （r =0. 999 8）. The average recovery rates were 99.8%, 100. 4% and 99.8%. The precision and accuracy of the assay were not more than 1.3%. Conclusion The method is rapid,accurate,reliable and can be used to control the quality of Qinhuanghouzheng Capsule.

关 键 词：黄芩苷 大黄素 大黄酚 芩黄喉症胶囊 高效液相色谱法 梯度洗脱 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]