反相高效液相色谱法测定妇康止痛片中大黄素含量  

Determination of Emodin in Fukangzhitong Tablets by RP-HPLC

作　　者：宋志军 

机构地区：[1]湖南省通道县民族中医院,湖南通道418500

出　　处：《中国药业》2008年第11期29-30,共2页China Pharmaceuticals

摘　　要：目的建立测定妇康止痛片中大黄素含量的反相高效液相色谱法。方法色谱柱为C18柱,流动相为甲醇-0.1%磷酸溶液(80∶20),检测波长为288nm,流速为1.0mL/min,柱温为25℃。结果大黄素进样量在0.05～0.40μg范围内与峰面积呈良好的线性关系,r=0.9995,平均加样回收率为98.54%,RSD=1.65%(n=6)。结论所用方法结果准确可靠,操作简便,可用于妇康止痛片的质量控制。Objective To develop a method for determination of emodin in Fukangzhitong Tablets by RP-HPLC. Methods The Cls column was used, The mobile phase was methanol-0.1% phosphoric acid （80：20）,The UV detector was set at the wavelength of 288 nm, The column temperature was 25 ℃ and the flow rate was 1.0 mL/min. Results The detectable content of emodin showed the good linear relationship in the range of 0.05 -0.40 μg（ r = 0. 999 5）, The average recovery rate of emodin was 98.54%, RSD = 1.65% （ n = 6）, Conclusion The method is simple,reliable, It can be used in the quality control of Fukangzhitong Tablets.

关 键 词：反相高效液相色谱法 妇康止痛片 大黄素 含量测定 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]