Electrochemical sensing of DNA immobilization and hybridization based on carbon nanotubes/nano zinc oxide/chitosan composite film  

作　　者：Wei Zhang Tao Yang Da Ming Huang Kui Jiao 

机构地区：[1]College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China

出　　处：《Chinese Chemical Letters》2008年第5期589-591,共3页中国化学快报（英文版）

基　　金：the National Natural Science Foundation of China(No.20635020,No.20375020);Doctoral Foundation of the Ministry of Education of China(No.20060426001).

摘　　要：A novel electrochemical DNA biosensor based on zinc oxide （ZnO） nanoparticles and multi-walled carbon nanotubes （MWNTs） for DNA immobilization and enhanced hybridization detection is presented. The MWNTs/nano ZnO/chitosan composite film modified glassy carbon electrode （MWNTs/ZnO/CHIT/GCE） was fabricated and DNA probes were immobilized on the electrode surface. The hybridization events were monitored by differential pulse voltammetry （DPV） using methylene blue （MB） as an indicator. The sensor can effectively discriminate different DNA sequences related to PAT gene in the transgenic corn, with a detection limit of 2.8× 10^-12 mol/L of target sequence.A novel electrochemical DNA biosensor based on zinc oxide （ZnO） nanoparticles and multi-walled carbon nanotubes （MWNTs） for DNA immobilization and enhanced hybridization detection is presented. The MWNTs/nano ZnO/chitosan composite film modified glassy carbon electrode （MWNTs/ZnO/CHIT/GCE） was fabricated and DNA probes were immobilized on the electrode surface. The hybridization events were monitored by differential pulse voltammetry （DPV） using methylene blue （MB） as an indicator. The sensor can effectively discriminate different DNA sequences related to PAT gene in the transgenic corn, with a detection limit of 2.8× 10^-12 mol/L of target sequence.

关 键 词：ZnO nanoparticles Multi-walled carbon nanotubes CHITOSAN DNA sensor PAT gene 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置] TB383[自动化与计算机技术—控制科学与工程]