萃取—紫外法测定乳香挥发油脂质体的包封率  

Determination of entrapment efficiency of Boswellia carterii Birdwood volatile oil lyposomes by extraction–UV

作　　者：刘让如[1] 谭桂山[2] 贺凌云[2] 肖娟[2] 

机构地区：[1]海南医学院药学系,海南海口571101 [2]中南大学药学院,湖南长沙410013

出　　处：《中国热带医学》2008年第6期1032-1033,共2页China Tropical Medicine

摘　　要：目的建立测定乳香挥发油（BCBV）脂质体包封率的方法。方法采用石油醚萃取-紫外法测定乳香挥发油脂质体的包封率。结果乳香挥发油在307nm附近有最大吸收峰，浓度在41．36～310．20μg·ml^-1范围内与吸光度A呈良好的线性关系，精密度良好（RSD=0．3％），低、中、高三个浓度的乳香挥发油溶液平均回收率均符合规定，分别为98．5％、99．3％、98．1％。结论各项方法学考察指标均符合规定，适用于乳香挥发油脂质体包封率的测定。Objective To establish a method for determination of the entrapment efficiency ofBoswellia carterii Birdwood volatile oil （to be abbreviated ： BCBV） liposomes. Methods Extraction - UV was adopted to measure the entrapment efficiency of BCBV liposomes. Results BCBV had the highest peak at 307nm in UV; a linear relationship was observed in the concentration rage of 41.36 -310.20μg·ml^-1; the precision was good（ RSD =0.3% ） ; the average recoveries of low ,middle and high concentrations were 98.5 %, 99.3% and 98.1%, respectively. Conclusion This method meets the requirements of methodologies and it is suitable for determination of the entrapment efficiency of BCBV hposomes.

关 键 词：乳香挥发油 脂质体 包封率 萃取 紫外法 

分 类 号：TQ646[化学工程—精细化工]