通心络对大鼠胚胎神经干细胞增殖和分化的影响  

Effect of Tongxinluo on the proliferation and differentiation of rat embryonic neural stem cells

作　　者：蒋海山[1] 李求实[1] 陆兵勋[1] 王立新[1] 尹瑞雪[1] 

机构地区：[1]南方医科大学南方医院神经内科,广东广州510515

出　　处：《南方医科大学学报》2008年第5期679-683,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(30400612)~~

摘　　要：目的探讨不同剂量通心络含药血清对大鼠胚胎神经干细胞增殖和分化的影响及其可能作用机制。方法自E12～14大鼠胚胎中分离、培养神经干细胞,添加不同剂量通心络含药血清干预,在干预后不同时段通过免疫荧光双标观察含药血清对大鼠胚胎神经干细胞增殖和分化的影响。结果大鼠胚胎神经干细胞经通心络含药血清干预后,Nestin(+)细胞比例先下降后回升,β-tubulin(+)细胞比例3d与7d时与对照组相比有显著性差异;7d时大剂量组β-tubulin(+)细胞比例与小剂量组相比有显著性差异。结论通心络可以促进大鼠胚胎神经干细胞的增殖及向神经元分化,大剂量组效应更明显及持久。Objective To investigate the effect of Tongxinluo in on the proliferation and differentiation of rat embryonic neural stem cells （NSCs）. Methods NSCs were isolated from 12- to 14-day SD rat embryo and treated with Tongxinluo at different doses, and the proliferation and differentiation of the cells were observed by immunofluorescence staining at different time points. Results The ratio ofembry0nic NSCs labeled with nestin decreased soon after Tongxinluo treatment, but increased afterwards. Significant difference was noted in the number of cells labeled with 13-tubulin between Tongxinluo group and the control group 3 and 7 days after the treatment, and also between high-dose and low-dose Tongxinluo groups at 7 days. Conclusion Tongxinluo can induce the proliferation and neuronal differentiation of rat embryonic NSCs, and the effect is related to the dose of Tongxinluo administered.

关 键 词：通心络 神经干细胞 细胞分化/药物作用 

分 类 号：R285.5[医药卫生—中药学]