支气管哮喘患者外周血中NK细胞含量的测定及临床意义  

Peripheral natural killer cell counting and its climical significance blood in patients with bronchial asthma

作　　者：刘芳[1] 罗雅玲[1] 吴月仙[2] 孔庆瑜[3] 

机构地区：[1]南方医科大学南方医院呼吸科,广东广州510515 [2]南方医科大学南方医院肺功能室,广东广州510515 [3]中山大学附属第一医院肾内科实验室,广东广州510080

出　　处：《南方医科大学学报》2008年第5期780-782,共3页Journal of Southern Medical University

基　　金：广东省科技项目(200334408);广州市科技计划(2004Z3-E0051)~~

摘　　要：目的观察支气管哮喘(哮喘)患者外周血中NK细胞含量的变化,探讨NK细胞与哮喘的发生是否相关。方法应用流式细胞仪检测128例哮喘患者:急性发作期(发作组)63例、哮喘缓解期(缓解组)65例及健康志愿者(正常对照组)62例外周血中NK细胞含量。结果急性发作期哮喘患者外周血中NK细胞含量(13.9±9.4)%,明显低于缓解组(22.5±12.3)%(P<0.01)和正常对照组(19.6±10.1)%(P<0.05)。缓解组与正常对照组比较无差异性(P>0.05)(F=4.695,P=0.012)。结论哮喘急性发作时外周血中NK细胞含量明显减少,提示NK细胞参与哮喘的发病过程。Objective To investigate the changes in natural killer （NK） cell count in the peripheral blood of asthmatic patients. Methods The number of NK cells in the peripheral blood was determined with flow cytometry in 63 asthmatic patients with acute episodes, 65 patients with stable asthma and 62 healthy nonatopic subjects. Results A significant decrease in NK cell number was noted in asthmatic patients during acute exacerbation [（13.9±9.4）%] in comparison with patients with stable asthma [（22.5±12.3）%] and healthy subjects [（19.6±10.1）%] （P〈0.05）, and the NK cell number showed no significant difference between the latter two groups （P〉0.05）. Conclusion NK cell number is reduced in acute exacerbation of asthma, suggesting its important role in the asthmatic process.

关 键 词：哮喘 NK细胞 流式细胞术 

分 类 号：R562.25[医药卫生—呼吸系统]