干扰素治疗慢性乙型肝炎患者肝组织细胞间粘附分子-1的表达  

Effects of interferon on hepatic intercellular adhesion molecule-1 expression in patients with chronic hepatitis B

作　　者：郑瑞丹[1] 徐成润[1] 张闽峰[1] 孟家榕[1] 饶日春[1] 

机构地区：[1]解放军第175医院肝病治疗中心,福建漳州363000

出　　处：《南方医科大学学报》2008年第5期878-879,882,共3页Journal of Southern Medical University

基　　金：南京军区卫生专业人才培养"122工程";福建漳州市科技基金(Z02050)

摘　　要：目的观察干扰素-α1b(IFN-α1b)治疗慢性乙型肝炎患者前、后肝组织细胞间粘附分子-1(ICAM-1)的表达及血清HBV DNA载量的变化。方法对慢性乙型肝炎患者治疗前及治疗结束时6个月行肝穿活检术,分别采用免疫组织化学法与实时荧光聚合酶链反应,检测肝组织ICAM-1的表达及血清中HBV DNA的载量变化。结果干扰素治疗前、后,慢性乙型肝炎重度与中度患者肝组织ICAM-1表达水平有显著差异(P<0.05);慢性乙型肝炎轻度患者肝组织ICAM-1表达水平无显著差异(P>0.05);治疗前肝组织ICAM-1染色强度(+)的患者,治疗前、后HBV DNA载量变化无明显差异(P>0.05);而肝组织ICAM-1染色强度(++)与染色强度(+++、++++)的患者,治疗前、后HBV DNA载量变化有差异(P<0.05,P<0.01)。结论IFN-α1b治疗慢性乙型肝炎患者疗效与肝组织表达ICAM-1有关,慢性乙型肝炎患者肝组织ICAM-1增强表达有利于IFN发挥抗病毒作用。Objective To investigate the effects of interferon -ctlb （IFN-αlb） on hepatic intercellular adhesion molecule-1 （ICAM-1） expression and serum HBV DNA in patients with chronic hepatitis B. Methods Before and 6 months after IFN-α lb treatment, liver biopsy was performed in patients with chronic hepatitis B to detect the expression of ICAM-1 in the liver tissues using immunohistochemistry. Serum HBV load was detected with real-time fluorescence polymerase chain reaction. Result CAM-1 expression in the liver tissue was significantly down-regulated after IFN treatment in patients with severe and moderate chronic hepatitis B （P〈0.05）. No significant variation was noted in the expression of ICAM-1 in the livers of patients with mild chronic hepatitis B after the treatment （P〉0.05）. In the patients weakly positive for ICAM-1 expression （＋）, serum HBV DNA varied scarcely after the treatment （P〉0.05）, while in the patients with strong ICAM-1 positivity （＋＋, ＋＋＋or ＋＋＋＋）, significant variation of serum HBV DNA occurred after the treatment （P〈0.05 or P〈0.01）. Conclusion The therapeutic effect of IFN-αlb is associated with the expression oflCAM-1 in the hepatocytes, and its expression might enhance the effects of IFN on HBV DNA in patients with chronic hepatitis B.

关 键 词：干扰素 肝炎 乙型 慢性 细胞间粘附分子-1 

分 类 号：R512.6[医药卫生—内科学]