桃核承气改良方对兔血管内膜剥脱术后内皮素和一氧化氮及一氧化氮合酶的影响  

Effects of improved prescription of Taoren Chengqi decoction on endothelin, nitric oxide and nitricoxide synthase after balloon angioplasty

作　　者：时广容[1] 宫丽鸿[1] 徐琳[1] 张雨[1] 

机构地区：[1]辽宁中医药大学,辽宁省沈阳市110032

出　　处：《中国组织工程研究与临床康复》2008年第20期3837-3840,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：辽宁省教委资助课题(05L246)~~

摘　　要：背景：大量的研究结果已证实，内皮细胞如能够早期覆盖受损的血管内膜层，则可避免血管内再狭窄的发生。目的：通过与西药作用对比，观察中药桃核承气改良方对兔血管损伤后内皮细胞生长及功能的影响。设计、时间及地点：随机对照动物实验，于2007—04／11在辽宁中医药大学和中国医科大学完成。材料：健康新西兰大耳白兔40只，体质量2．0～2．5kg，高酯饲料喂养4周行腹主动脉内膜剥脱造模，制备动脉粥样硬化模型。桃核承气改良方为辽宁中医药大学附属第一医院制剂产品；舒降之为杭州默沙东制药有限公司产品；阿司匹林为拜尔药业集团有限公司产品。方法：40只兔随机数字表法分为4组。造模后模型组、中药组、西药组正常饲料喂养，中药组予桃核承气改良方13．5mL／kg灌胃4周，西药组予舒降之、阿司匹林混合液13．5mL／kg灌胃4周，模型组、空白组予生理盐水13．5mL／kg灌胃4周。主要观察指标：①造模后1，28d采用硝酸还原法法和比色法测定血浆内皮素1、血清一氧化氮含量。②免疫组织化学法测定血管中内皮素1、内皮型一氧化氮合酶蛋白表达。结果：32只新西兰大耳白兔进入结果分析，模型组（n=8）、中药组（n=10）、两药组（n=8）和空白组（n=6）。①与空白组相比，其他3组造模后1d血浆内皮素1升高，血清一氧化氮含量下降（P〈0．05）。造模后28d兔中药组、西药组血浆内皮素1较模型组明显降低，血清一氧化氮明显升高（P〈0．05）。②血管中内皮素1表达模型组明显高于其他3组（P〈0．05）；中药组、西药组较模型组显著减弱（P〈0．05）。模型组内皮型一氧化氮合酶表达明显低于其他3组（P〈0．05）；中药组、西药组较模型组显著增强（P〈0．05）。结论：桃核承气改良方能够促进内皮素与一氧化氮之间的平衡协调，有利�BACKGROUND： Plenty of studies have demonstrated that early growth of endothelial cells to injured tunica intima can prevent intravascular restenosis. OJECTIVE： To investigate the effects of improved prescription of Taoren Chengqi decoction （Peach Kernel decoction for strengthening qi） on the growth and activation of endothelial cells in rabbits after balloon angioplasty. DESIGN, TIME AND SETTING： Randomized controlled animal trial was performed at the Liaoning University of Traditional Chinese Medicine and China Medical University from April to November 2007. MATERIALS： Forty healthy New Zealand White Rabbits weighing 2.0-2.5 kg were fed with high fat diet for 4 weeks, and created models of artherosclerosis by stripping abdominal aortic tunica intima. The improved prescription of Taoren Chengqi decoction was product of the First Hospital of Liaoning University of Traditional Chinese Medicine; Zocor （simvastatin） is product of Hangzhou Moshadong Pharmaceutical Co., Ltd., Zhejiang Province; aspirin was product of Bayer. METHODS： Forty rabbits were randomly divided into model group （n=11）, Chinese medicine group （n=12）, western medicine group （n=11 ）, and blank group （n=6）. After modeling, the model group, Chinese medicine group, western medicine group were fed with normal diet. Additionally, the Chinese medicine group was intragastrically infused improved prescription of Taoren Chengqi decoction （13.5 mL/kg） for 4 weeks; the western medicine group was infused with simvastatin and aspirin mixture （13.5 mL/kg） for 4 weeks; the model group and blank group were infused with normal saline （13.5 mL/kg） for 4 weeks. MAIN OUTCOME MEASURES： The levels of plasma endothelin-1 （ET-1） and serum nitric oxide （NO） were measured by radio-immunity and colorimetry; vascular ET-1 and nitricoxide synthase （NOS） protein expressions were determined using immunohistochemical method. RESULTS： Thirty-two rabbits were included in the final analysis. Compared with the blan

关 键 词：桃核承气改良方 血管内膜剥脱术 内皮素1 一氧化氮 内皮型一氧化氮合酶 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]