兔自体移植静脉内膜增生与缬沙坦的干预效应  

Inhibitory effect of valsartan on intimal hyperplasia in rabbit autogenous grafted veins

作　　者：仲崇俊[1] 蒋庚西[1] 

机构地区：[1]南通大学第二附属医院心胸外科,江苏省南通市226001

出　　处：《中国组织工程研究与临床康复》2008年第20期3859-3862,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：南通市社会发展计划资助项目(S2005023)~~

摘　　要：目的：内膜增生是移植静脉再狭窄的主要原因，血管紧张素Ⅱ在血管组织增生中发挥着重要作用，缬沙坦作为AT1受体拮抗剂可在受体水平阻断血管紧张索Ⅱ的生物学作用。实验拟验证AT1受体拮抗剂缬沙坦对兔颈部静脉移植术后血管内膜增生的干预作用。方法：实验于2005-10／2006—10在南通大学动物实验中心完成。①实验分组：雄性新西兰大白兔20只，微生物控制级别Ⅰ级，兔龄16～18周，体质量（2．5±0．5）kg，随机排列表法分为对照组和治疗组，每组10只。②实验方法：所有动物行颈部自体静脉移植手术，对照组予普通饲料饲养；治疗组则予普通饲料＋缬沙坦[10mg/（kg·d）]喂养。③实验评估：饲养4周后观察两组动物治疗前后的血压变化情况：取出静脉移植物行病理形态学检查；采用图像分析系统计算血管腔内膜的厚度、面积及中膜的厚度、面积：免疫组织化学方法检测增殖细胞核抗原的表达。结果：纳入大白兔20只，均进入结果分忻，术后切口无感染，两组移植静脉无闭塞。①对照组和治疗组动物的血压在治疗前后无明显变化（P〉0.05）。②对照组移植物内膜明显增厚，内膜下大量平滑肌细胞增殖，中膜稍有增厚：治疗组内膜增厚较对照组减弱，中膜平滑肌细胞向内膜下迁移、增殖较对照组明显减轻，中膜增厚不明显。③治疗组新生内膜厚度、面积及增殖细胞核抗原均低于对照组（P〈0.01）。结论：缬沙坦可以显著抑制兔自体静脉移植后的再狭窄，并抑制移植静脉桥中增殖细胞核抗原的表达。AIM： Intimal hyperplasia （IH） is the main cause of the restenosis of grafted veins. Angiotensin Ⅱ plays an important role in vascular tissue hyperplasis. Valsartan, AT1 receptor antagonist, can inhibit the biological effect of angiotensin Ⅱ. This study investigated the inhibitory effect of valsartan on IH in rabbit autogenous grafted veins. METHODS： The experiment was performed in Animal Experiment Center of Nantong University from October 2005 to October 2006. Twenty male New Zealand white rabbits of 16-18 weeks old and （2.5 ±0.5） kg were randomly divided into control group and treatment group （n=10）. All rabbits underwent autogenous vein grafting. The control group was fed with common diet, whereas the treatment group was fed with an admixture of valsartan （10 mg/kg · d ） and common diet. Four weeks after operations, the mean arterial pressure （MAP） of the rabbits were monitored before and after operation. The grafted veins were harvested for pathomorphologic examinations; the thickness and area of the intima and media of the veins were calculated with Image Analyzer System; the expression of proliferating cell nuclear antigen （PCNA） in veins were examined with immunohistochemistry. RESULTS： Twenty rabbits were involved in the result analysis. There was no wound infection in rabbits and none of the vein grafts was occluded. No significant difference was observed between pre- and post-treatment MAP in both groups （P 〉 0.05）. In the control group, the intima of grafted veins were thickened evidently with proliferated vascular smooth muscle cells （VSMCs） and the media thickness were slight. Compared with the control group, the intima was slightly thickened in the treatment group, but there were significant reductions in proliferated VSMCs. Additionally, the media thickness was unobvious in treatment group. Compared with the control group, the thickness and area of neointima and the expression of PCNA in the treatment group were significantly lower than those in the

关 键 词：AT1受体拮抗剂 自体移植静脉 增殖细胞核抗原 

分 类 号：R622.4[医药卫生—整形外科]