钙调蛋白在氟中毒大鼠肝组织中的表达  

Expression of calmodulin in hepatocytes of rats with fluorosis

作　　者：吕学霞[1] 于燕妮[1] 陈杨[1] 万昌武[1] 张华[1] 官志忠[1] 

机构地区：[1]贵阳医学院病理教研室,贵州550004

出　　处：《中国地方病防治》2008年第3期165-167,共3页Chinese Journal of Control of Endemic Diseases

基　　金：贵州省国际科技合作重点项目;编号:黔科合外G字(2007)400119号;国家重点基础研究发展计划(973计划)课题;黔科合重大专项字[2006]6015

摘　　要：目的探讨钙调蛋白在氟中毒大鼠肝组织中的表达及意义。方法在饮水中加入氟化钠进行大鼠染毒试验,3个月后采用免疫组化技术及RT-PCR技术检测大鼠肝脏中钙调蛋白(CaM)的表达水平。结果与对照组相比,染氟浓度为25mg/L、50mg/L、100mg/L组大鼠肝脏CaM mRNA及蛋白表达水平均增高,并随染氟浓度的增加,表达增强。结论氟可以导致大鼠肝脏CaM mRNA及蛋白表达水平增强。Objective To investigate the meaning of calmodulin in hepatocytes of rats with fluorosis. Methods Experimental Wistar rats were exposed to sodium fluoride （NaF） added to drinking water. Expression of both mRNA and protein of calmodulin in rat liver tissue were determined by RT - PCR and immunohistochemistry after three - month experimental period. Results The results showed that the expression of calmodulin mRNA and protein in those groups treated with fluoride （ concen-trations of 25 mg/L,50 mg/L and 100 mg/L） were increased. And the higher the concentration was, the higher the expression level was. Conclusions Fluoride can lead to the high expression of calmodulin mRNA and protein in the rat liver tissue exposed to sodium fluoride.

关 键 词：钙调蛋白 氟中毒 免疫组化 RT-PCR 

分 类 号：R516.8[医药卫生—内科学]