小鼠脾脏来源的调节性表型γδT细胞的存在和体外诱导  

Existence and in vitro induction of mouse spleen derived γδT cells with regulatory phenotypes

作　　者：杨静琳[1] 康宁[2] 汤龙[2] 陈兴明[3] 刘庆丰[2] 崔莲仙[2] 胡云章[1] 何维[2] 

机构地区：[1]北京协和医学院医学生物研究院中国医学科学院医学生物研究所疫苗室,昆明650118 [2]北京协和医学院基础学院,中国医学科学院基础医学研究所免疫学系,医学分子生物学国家重点实验室,北京101005 [3]北京协和医院耳鼻喉科,北京101005

出　　处：《中国免疫学杂志》2008年第6期488-491,共4页Chinese Journal of Immunology

基　　金：国家973免疫学项目(2007CB512405);北京协和医院基础与临床合作项目(2006407)

摘　　要：目的:研究初始γδT细胞是否存在具有调节细胞表型的亚群,同时是否能够像CD4+CD25-T细胞一样可以在体外诱导产生具有调节表型的γδT细胞。方法:采用流式分选或磁珠分选的方法分离纯化脾淋巴细胞中γδT细胞,通过反转录聚合酶链反应(RT-PCR)、实时定量PCR(Real-time PCR)和免疫荧光染色分析初始的γδT细胞以及TGFβ-(5ng/ml)诱导后的γδT细胞中Foxp3及部分调节性T细胞表面分子和细胞因子的表达情况。结果:与CD4+CD25+T细胞相比,初始的γδT细胞Foxp3表达量很低;活化的γδT细胞经过TGFβ-诱导后Foxp3表达量明显增加,同时与免疫调节相关的表面分子如GITR、CTLA-4和细胞因子TGFβ-、IL-10的表达量也相应增加,IFNγ-的表达有所降低。结论:初始的γδT细胞低表达Foxp3,在体外可经TGFβ-诱导产生具有调节性T细胞表型的亚群。Objective：To study whether a subpopulation of naive γδT cells with regulatory phenotype exists in murine spleens, and whether it can be induced in vitro as CD4^＋ CD25^- T cells likewise.Methods： γδT cells were isolated by Fluorescent activated cell sorting or magnetic cell sorting from mouse spleen. Reverse transeriptase PCR （ RT-PCR）, Real-time PCR and immunofluorescence staining methods were used to detect the expression of Foxp3 and some immunoregulation-related surface molecules and cytokines in freshly isolated and TGF-β （5 ng/ ml） induced γδT cells. Results： Compared with that of CD4^＋ CD25^＋ T cells, the expression level of Foxp3 in naive γδT cells was much lower. However, following TGF-β induction, the expression level of Foxp3 and immunoregulation-related surface molecules and cytokines such as GITR,CTLA-4,TGF-β and IL-10 increased,whereas IFN-γ expression level decreased in activated γδT cells. Conclusion：The freshly isolated γδT cells express low level of Foxp3. TGF-β induction of activated γδT cells in vitro could generate a subpopulation with regulatory T cell phenotype.

关 键 词：ΓΔT细胞 调节性T细胞 FOXP3 免疫调节 

分 类 号：R12[医药卫生—环境卫生学]