云芝糖肽、丹参酮ⅡA对荷瘤小鼠的抗肿瘤及免疫调节作用  

Anti-tumor effects and immunoregulatory activity of Polysaccharopeptide from Yunzhi and TanshinoneⅡA in EAC tumor-bearing mice

作　　者：祝绚[1] 鲍依稀[1] 李进[1] 刘靖 王宏萍[1] 

机构地区：[1]重庆医科大学免疫学教研室临床检验诊断学省部共建教育部重点实验室,重庆400016

出　　处：《中国免疫学杂志》2008年第6期526-529,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金(30471837);重庆市科委基金(CSTC;2006BB5039);重庆市教委基金(KJ060307);校办启动基金(QD200540)资助课题

摘　　要：目的:研究云芝、丹参有效组分单体以及复方对EAC荷瘤小鼠的抗肿瘤和免疫调节作用,为肿瘤的中药治疗新方剂的临床广泛应用提供一定的实验基础。方法:建立EAC实体型荷瘤小鼠动物模型,随机分为6组:生理盐水对照组(NS)、阿霉素治疗组(ADM)、云芝糖肽组(PSP)、丹参酮Ⅱ组(TanⅡA)、复方组(PSP+TanⅡA)和综合治疗组(PSP+TanⅡA+ADM),并给予相应药物治疗,应用免疫组化法检测小鼠脾脏IL-2、IL-2R和肿瘤组织中周期蛋白CDK4以及凋亡蛋白Bax、Bcl-2表达,并计算抑瘤率、脏器指数,结果与对照组和化疗组进行比较。结果:云芝糖肽组小鼠脾脏IL-2、IL-2R水平高于NS组和化疗组(P<0.05),各药物治疗组肿瘤Bax均高于化疗组,CDK4均低于NS组(P<0.05),复方组Bax高于NS组(P<0.05),云芝组糖肽、丹参酮ⅡA组、综合治疗组CDK4低于化疗组(P<0.05),云芝糖肽组、复方组与综合治疗组肿瘤Bcl-2低于NS组(P<0.05),各药物治疗组对肿瘤有明显的抑制作用(P<0.05),复方组胸腺指数高于NS组,各药物治疗组胸腺指数均高于化疗组(P<0.05)。结论:云芝糖肽、丹参酮ⅡA对EAC荷瘤小鼠有明显的抗肿瘤和免疫调节作用,并能缓解化疗药物所引起的毒副作用,二者联用存在一定协同作用。Objective：To study the anti-tumor effects and immtmoregulatory activity of effective ingredients in Yunzhi （PSP） and Danshen （TanⅡ A） by a model of EAC tumor-beating mice,and to provide the fundament to tumor therapy with the traditional Chinese medicines alone and in combination. Methods： Mice were inoculated with EAC ceils and randomized into 6 groups： the control group, ADM group,PSP group,Tan Ⅱ A group,PSP ＋ TanⅡ A group and PSP ＋ TanⅡ A ＋ ADM group. PSP and TanⅡ A were used in alone or in combination. Immunohistochemical methods were employed for detection of IL-2, IL-2R in spleens and CDK4, Bax/Bcl-2 in the tumor tissues of tumor-beating mice. The inhibition rates of tumor growth and thymus index of the therapy groups with the traditional Chinese medicines were calculated and compared with control and ADM groups. Results：The level of IL-2 and IL-2R expressed in spleens was obviously higher in PSP than in both control and ADM group（ P 〈 0.05）. Expressing level of Bax in tumors, particularly in the PSP ＋ Tan Ⅱ A group, was obviously increased in all the therapy groups than in ADM group （ P 〈 0.05 ） and the control group. CDK4 expression in the tmnor was obviously decreased in the thempy groups vs the control group（ P 〈 0.05 ）, especially in PSP group, Tan Ⅱ A group and PSP ＋ Tan Ⅱ A ＋ ADM group in which CDK4 expression was lower than in ADM group（ P 〈 0.05 ）. Bcl-2 expression in the tumor was obviously decreased in PSP group, PSP ＋ Tan Ⅱ A group and PSP ＋ Tan Ⅱ A ＋ ADM group than in the control group （P 〈 0.05 ）. The growth of implanted tumor was significantly inhibited by the Chinese medicines and chemotherapeutic drugs（ P 〈 0.05 ）. Thymus index of PSP ＋ Tan Ⅱ A group was significantly increased vs control group （ P 〈 0.05） ;And thymus index in all therapeutic groups was significantly higher than in ADM control （P 〈 0.05）. Conclusion： PSP and Tan Ⅱ A have inhibitory effects on the in vivo propagation

关 键 词：云芝糖肽 丹参酮ⅡA 艾氏腹水癌 免疫调节 周期蛋白 凋亡相关蛋白 

分 类 号：R285.5[医药卫生—中药学] R286.91[医药卫生—中医学]