遵义地区汉族HLA-DRB1、DQB1等位基因多态性的研究  

Research on genetic polymorphisms of HLA-DRB1 and DQB1 in the Hans of Zunyi

作　　者：罗军敏[1] 孙万邦[1] 黄学贵[2] 冯继红[1] 张忆雄[1] 姚新生[1] 宋明英[1] 

机构地区：[1]遵义医学院免疫学教研室,遵义563003 [2]遵义医学院寄生虫学教研室,遵义563033

出　　处：《中国免疫学杂志》2008年第6期542-544,548,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金资助项目(项目号:30360099)

摘　　要：目的:从基因水平调查遵义地区汉族人群HLA-DRB1、DQB1等位基因频率,并了解其多态性分布状况。方法:应用聚合酶链反应-序列特异性引物(PCR-SSP)方法对遵义汉族200名健康个体进行HLA-DRB1、DQB1等位基因分型。结果:遵义地区汉族HLA-DRB1、DQB1等位基因频率分布,在中低分辨水平,共检出13个DRB1基因,7个DQB1基因,DRB1*09、DRB1*08及DQB1*05基因频率相对较高;DRB1*10及DQB1*04基因频率相对较低。与我国北方、南方汉族比较,更接近南方汉族人群。结论:遵义汉族人群HLA-DRB1、DQB1基因具有较为丰富的多态性,其分布符合南方汉族的特点,可能与重庆汉族人群有较为密切的民族融合。Objective：To investigate frequencies and polymorphism of HLA-DRB1 and DQB1 allele in the Hans of Zunyi area.Methotis： Polymerase chain reaction-sequence specific primers （PCR-SSP） were used to type HLA-DRB1 and DQB1 genes of 200 unrelated healthy Han individuals in Zunyi area. Results： 13 HLA-DRB1 and 7 HLA-DQB1 alleles were obtained at low resolution level in all subjects. The allele DRB1 ＊ 09, DRB1 ＊ 08 and DQB1 ＊ 05 were showed high distributing frequencies;The allele DRB1 ＊10 and DQB1 ＊04 were scarcely found with low distributing frequencies. Comparied with Northern and Southern Han people, it would seem that Han people in Zunyi are more closely related to the Southern ones. The allele B ＊ 07 was scarcely found in the Southern Han with a high distributing frequency （ GF = 2.0% ）. Conclusion： HLA-DRB1 and DQB1 of Han people in Zunyi have plenty of polymorphisms. They seem to distribute in line with the Southern Han＇s characteristics but have their own territory feature with a high B ＊ 07 frequency.

关 键 词：遵义 HLA-DRB1、DQB1 基因频率 多态性 聚合酶链反应-序列特异性引物(PCR-SSP) 

分 类 号：R392.2[医药卫生—免疫学]