青霉素特异性IgE抗体的种类与HLA-DRB基因多态性  

Correlation of HLA-DRB genetic locus with penicillin-specific IgE antibodies

作　　者：杨静[1] 乔海灵[1] 

机构地区：[1]郑州大学临床药理研究所,河南郑州450052

出　　处：《中国药理学通报》2008年第6期777-781,共5页Chinese Pharmacological Bulletin

基　　金：河南省杰出青年基金资助项目(No0312002100);河南省自然科学基金资助项目(No0211040100);河南省医学创新人才工程资助项目(No2002114)

摘　　要：目的探讨HLA-DRB基因是否调控青霉素过敏反应产生不同种类的IgE抗体。方法以河南汉族临床青霉素过敏反应的病人为研究对象,采用放射免疫吸附试验(radio allergosorbent test,RAST)检测血清中针对青霉素药物的8种特异性IgE抗体和采用序列特异性引物-聚合酶链反应(pol-ymerase chain reaction sequence-specific primer,PCR-SSP)的方法检测HLA-DRB等位基因。结果80例抗体阳性病人中,BPO、BPA、PVO、PVA、APO、APA、AXO、AXA-IgE阳性的分别为36、33、32、29、21、12、16、33例。与对照组相比,BPO-IgE阳性组DR16基因和PVO阳性组DR14.1基因明显降低(0vs8.62%,P<0.01;1.56%vs9.77%,P<0.05)。结论DR16基因可能与BPO抗原的过敏有关,DR14.1基因可能与PVO抗原的过敏有关。Aim To explore the correlation of positive specific IgE antibodies with penicillin allergy and HLA-DRB genetic polymorphism. Methods Sequence specific primer-polymerase chain reaction （PCR-SSP） was used to type human leucocyte antigen-DBR（ HLA- DRB） alleles in 80 patients with positive IgE antibod- ies to penicillin and 101 healthy subjects without any allergic reactions. Results When different penicillin antigens were analyzed separately ,the gene frequencies of DR16 in patients with positive BPO-IgE antibody and DR14. 1 in patients with positive PVO-IgE antibody were found greatly lower than those of the control subjects（ 0 vs 8.62% ,P 〈0. 01 ;1.56% vs 9.77% ,P 〈 0. 05 ）. Conclusions DR16 gene and allergy to BPO antigen were correlated, and the same was true between DR14. 1 gene and allergy to PVO antigen.

关 键 词：过敏反应 青霉素 基因多态性 HLA-DRB IGE 

分 类 号：R392.11[医药卫生—免疫学] R392.2[医药卫生—基础医学]