多色荧光原位杂交检测弱精子症的非整倍体  

Aneuploidy Detection by Multi-color Fluorescence in Situ Hybridization in Patients with Asthenospermia

作　　者：马明福 李练兵 吕静 李新生 陈晓星 赵乐天 

机构地区：[1]重庆市人口和计划生育科学技术研究院,重庆400020

出　　处：《癌变．畸变．突变》2008年第3期239-241,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：重庆市人口和计划生育委员会课题资助(004002)

摘　　要：背景与目的:以多色荧光原位杂交技术评价弱精子症患者的精子非整倍体。材料与方法:应用X、Y、18号染色体探针对6例弱精子症患者和3例健康男性进行多色荧光原位杂交试验,检测精子的非整倍体。结果:计数30305个精子,杂交率99.98%。缺体类型主要为XX18、YY18、XY18、X1818、Y1818。实验组缺体率分别为0.133%±0.095%、0.135%±0.231%、0.438%±0.415%、0.507%±0.973%及0.432%±0.705%,对照组缺体率分别为0.011%±0.020%、0.006%±0.010%、0.051%±0.075%、0.023%±0.040%及0.034%±0.034%。实验组的非整倍体率为2.02%,对照组为0.25%,二者比较,差异具有统计学意义(P<0.01)。结论:多色荧光原位杂交技术对于准确检测人精子非整倍体具有较高的应用价值。BACKGROUND AND AIM： Multi-color fluorescence in situ hybridization（MFISH） was employed to evaluate the frequency of aneuploid sperm in patients with asthenospermia. MATERIALS AND METHODS：Patients were divided into 2 groups： 6 with asthenospetmia and 3 normal healthy men used as control. MFISH and probes for chromosomes including X,Y and 18 were used to determine the frequency of aneuploidy in sperms. RESULTS： Analysis was performed on 30 305 sperms. The rate of hybridization was 99.98%. Nullisomy kinds included XX18, YY18, XY18, X1818, Y1818. Nullisomy frequencies were 0. 133%% ± 0.095% ,0. 135%±0.231% ,0.438%±0.415% ,0.507% ± 0.973% ,0.432% ±0.705%, respectively, and were 0.011% ±0.020%, 0.006%±0.010%, 0.051% ±0.075%, 0.023%±0.040%, 0.034% ± 0.034% in control group. Aneuploidy in the sperms of test group and control group were 2.02% and 0.25%, respectively, significantly higher in the test group（P 〈0.01） .CONCLUSION： Sperm aneuploidy can be reliably determined by sperm MFISH analysis in men with asthenospermia.

关 键 词：弱精子症患者 多色荧光原位杂交 非整倍体 

分 类 号：R698[医药卫生—泌尿科学]