人巨细胞病毒感染对宿主细胞CyclinE/Cdk2激酶活性影响的实验研究  

The influence of human cytomegalovirus infection on activity of cyclin E/Cdk2 in host cell

作　　者：刘楠[1] 

机构地区：[1]南方医科大学附属南方医院妇产科,广州510515

出　　处：《中国优生与遗传杂志》2008年第6期36-37,共2页Chinese Journal of Birth Health & Heredity

基　　金：国家自然基金资助项目(项目批准号30271673)

摘　　要：目的研究HCMV感染对Cdk2蛋白水平及对CyclinE/Cdk2激酶活性的影响。方法通过接触抑制使细胞同步化于G0/G1期,用MOI=5PFU/per cell HCMV AD169毒株感染人胚肺成纤维细胞(HEL);用免疫沉淀,激酶活性分析法检测HCMV感染细胞Cdk2的活性。结果HCMV感染可引起CyclinE/Cdk2激酶的强烈激活,但HCMV感染并不诱导Cdk2蛋白丰度增加。结论HCMV感染G0/G1细胞,激活CyclinE/Cdk2激酶。使细胞周期越过G1/S限制点,进展至晚Gl期。Objective： To investigate the effect of HCMV infection on Cdk2 protein level and activity of cyclin E/Cdk2. Methods： The abundance of Cdk2 protein determined by Western blot, the activity of Cdk2 assayed by immunoprecipitation and kinase assay. Results： In this report, we demonstrated that HCMV infection results in robust activation of cyclinE/Cdk2 kinase. However HCMV infection can not induce the increase of the abundance of Cdk2 protein. Conclusion ： The G0/G1 cells infected by HCMV lead to robust activation of cyclinE/Cdk2 kinase. All the above leads to skip G1/S restriction points and cell cycle progresses to later G1.

关 键 词：HCMV CyclinE/Cdk2激酶 免疫沉淀 激酶活性分析法 

分 类 号：R373.4[医药卫生—病原生物学]