STEM-X的急性毒性与抗肿瘤活性实验研究  

An Experimental Study of Acute Toxicities and Anti-tumor Activity of STEM-X

作　　者：陈云舟[1] 杜光[1] 宗凯[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院药学部,武汉430030

出　　处：《医药导报》2008年第6期623-624,共2页Herald of Medicine

摘　　要：目的研究STEM-X的急性毒性和体内对肿瘤的抑制作用。方法采用急性毒性实验:选取昆明种小鼠30只,随机分为3组,每组10只。禁食24h后一次性给药。给药后连续观察14d,详细记录小鼠的死亡数目、死亡时间及主要毒性反应。抗肿瘤实验:建立S180荷瘤小鼠模型40只,随机分为4组,每组10只,即低剂量药物组(浓缩液10mL.kg-1),高剂量药物组浓缩液(20mL.kg-1),氟尿嘧啶(5-Fu)阳性对照组(20mg·kg-1),空白对照组。连续灌胃给药10d,处死动物,称重,完整剥离瘤块并称瘤重。计算肿瘤抑制率。结果急性毒性实验的3个剂量组均未出现小鼠死亡,无明显异常。STEM-X浓缩液的半数致死量(LD50)>50mL.kg-1。抗肿瘤活性实验中,高、低剂量组、阳性对照组的瘤重和空白组比较,差异有显著性。高剂量组的肿瘤抑制率和阳性对照组比较,差异无显著性。低剂量组和阳性对照组比较,差异有显著性。结论STEM-X在正常剂量下,安全、毒副作用极低。对S180肉瘤有明显抑制作用。Objective To study the acute toxicities and anti-tumor activity of STEM-X in vivo. Methods The acute toxicity of STEM-X was assayed as followed ： thirty mice were averagely divided into three groups. After 24 hours fasting, STEM- X was given to the mice. The numbers and time of death mice, and primary toxic symptom were observed. The anti-tumor activity of STEM-X was research as followed： forty mice were randomly divided to four groups （ each group with 10 mice） ： low dose group （ concentrated STEM-X 10 mL·kg^-1 ）, high dose group （ concentrated STEM-X 20 mL·kg^-1 ）, 5-Fu control group （20 mg·kg^-1） and blank control group and transplanted with the sarcocarcinoma 180 according to a standard method. The STEM-X solution was given to mice for 10 days by i. g route. Results No dead mouse was observed in three STEM-X groups in acute toxicity assay. The LD50 of concentrated STEM-X was over than 50 mL·kg^-1. For the anti-tumor activity of STEM-X, the tumor weight in high dose group and 5-Fu control group is obviously different from that in control group. The inhibitory rate has no marked difference between high dose group and 5-Fu control group, but is higher than that in low dose group. Conclusion In the normal dose, STEM-X is a safe agent with lowly toxicity and shows an obvious anti-tumor activity to sarcocarcinoma 180.

关 键 词：STEM—X 毒性 急性 S180 肿瘤抑制率 

分 类 号：R282.71[医药卫生—中药学] R285.5[医药卫生—中医学]