参一胶囊联合顺铂对高转移人卵巢癌细胞系HO-8910PM的抑制作用  

The Suppression Effect of Shenyi Capsule on Human High-metastasis Ovarian Cancer Cell Line HO-8910PM

作　　者：陈鲁[1] 朱笕青[1] 钱丽娟[2] 顾琳慧[2] 郑智国[2] 马胜林[2] 夏婷[1] 

机构地区：[1]浙江省肿瘤医院妇瘤科,杭州310022 [2]浙江省肿瘤研究所,杭州310022

出　　处：《医药导报》2008年第6期637-639,共3页Herald of Medicine

基　　金：浙江省中医药科技计划基金资助项目(基金编号:2005-136B)

摘　　要：目的观察参一胶囊(Rg3)对高转移人卵巢癌细胞系HO-8910PM的抑制作用。方法细胞分成6组:阴性对照组(A组):只加新鲜的全培养液;低浓度组(B组):含10μg.mL-1Rg3培养液;中浓度组(C组):含20μg·mL-1Rg3培养液;高浓度组(D组):含40μg·mL-1Rg3培养液;顺铂阳性对照组(E组):含10μg·mL-1顺铂的培养液;顺铂+Rg3联合用药组(F组):含10μg·mL-1顺铂+20μg·mL-1Rg3培养液。分别从台盼蓝活细胞计数法、细胞的增殖抑制(CCK-8法)进行观察。结果Rg3对人卵巢癌HO-8910PM细胞有明显的抑制作用,显示了较好的细胞毒活性作用(P<0.05)。Rg3与顺铂联合用药时,细胞毒作用与高浓度Rg3和顺铂组相似,但在微观结构上能造成更大的细胞形态结构损伤。结论Rg3对高转移人卵巢癌细胞有一定的抑制作用,Rg3与顺铂联合用药在细胞结构破坏上可能有相加或协同作用,这将为临床卵巢癌患者进行抗肿瘤治疗提供依据。Objective To study the suppression effect of shenyi capsule(ginsenoside Rg3)combined with cisplatin on high-metastasis human ovarian cancer cell line HO-8910PM.Methods The cell line was divided into 6 groups randomly:negative control group(A);low concentration Rg3(10 μg·mL-1)group(B);middle concentration Rg3(20 μg·mL-1)group(C);high concentration Rg3(40 μg·mL-1)group(D);cisplatin(10 μg·mL-1)positive control group(E);combination of cisplatin+Rg3 group(F):10 μg·mL-1 cisplatin +20 μg·mL-1 Rg3.CCK-8 method and microscopic observation were used to investigate the suppression effects.Results Rg3 has a significant cytotoxic effect to human ovarian cancer cell,especially under the high concentration(P<0.05).Combination of Rg3 and cisplatin has the similar anti-tumor effect to D group,but shows a stronger injury in the morphology and structure of cell.Conclusion Rg3 has the anti-tumor effect with human ovarian cancer cell line HO-8910PM and combination of Rg3 and cisplatin may be a more appropriate strategy.

关 键 词：参一胶囊 卵巢癌 HO-8910PM细胞系 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]