平肝潜阳法对偏头痛肝阳上亢型大鼠模型肾上腺组织蛋白质组的影响  

Effects of ping-gan qianyang therapy on adrenal gland proteome in migraine rat model with hyperactivity of liver-yang

作　　者：胡建军[1] 陈泽奇[1] 钟广伟[1] 李炜[1] 尹耀慧[1] 

机构地区：[1]中南大学湘雅医院中西医结合研究所,湖南长沙410008

出　　处：《中国医师杂志》2008年第5期590-593,共4页Journal of Chinese Physician

基　　金：国家自然科学基金（青年基金）资助（30500644,30472115）;湖南省重点学科资助项目（湘教通2001-179）

摘　　要：目的探讨平肝潜阳法对偏头痛肝阳上亢型大鼠模型作用的分子机制。方法用电刺激SD大鼠三叉神经节的方法并灌服附子汤制造偏头痛肝阳上亢型模型。采用双向凝胶电泳（2-DE）技术分离大鼠肾上腺组织的总蛋白，图像分析识别差异表达的蛋白质点，基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）获得相应的肽质量指纹图（PMF），搜索数据库鉴定蛋白质。结果得到分辨率及重复性较好的正常对照组、偏头痛肝阳上亢型大鼠、中药治疗组大鼠肾上腺组织的2-DE图谱，质谱分析鉴定得到其中的8个差异蛋白质点。结论平肝潜阳药物可能通过调节多种与能量代谢有关的蛋白和泛素的表达而起到治疗偏头痛肝阳上亢证的作用。Objective To explore the molecule mechanisms of ping-gan qianyang therapy on migraine rat model with the hyperactivity of liver-yang. Methods An animal model of migraine with hyperactivity of liver-yang was established through electrical trigeminal gangli- on stimulation and administering orally with Fuzi decoction. The total proteins of adrenal gland in the rats were separated by immobilized PH gradient-based two-dimensional gel electrophoresis, and the 2-DE images was analyzed by PDQuest 7. 0 software. Matrix-Assisted Laser Desorption/Ionization-Time Of Flight Mass Spectrometry （MALDI-TOF-MS） and SWISS-PORT and MSDB database were used to identify differ- ential proteins. Result The well-resolution and reproducible 2-DE patterns of rat adrenal glands in normal group, model group and therapy group were obtained A total of 8 differentially expressed proteins were identified. Conclusion Ping-gan qianyang therapy may effect on migraine by regulating the expression of proteins related to energy metabolism and ubiquitin.

关 键 词：偏头痛 蛋白质组学 肾上腺 中药疗法 

分 类 号：R277.7[医药卫生—中医学]