脂蛋白（a）对大鼠肾小球系膜细胞细胞间黏附因子-1表达的影响  

Regulation of intercellular adhesion molecule-1 expression in murine glomerular mesangial cells by lipoprotein（a）

作　　者：向伟[1] 何小解[2] 谢慧能[1] 易著文[2] 何庆南[2] 吴小川[2] 符生苗[1] 陈炽[1] 王福利[1] 

机构地区：[1]海南省人民医院儿科,海南海口570311 [2]中南大学湘雅二医院小儿肾脏病研究室

出　　处：《中国医师杂志》2008年第5期603-606,共4页Journal of Chinese Physician

基　　金：海南省卫生厅资助课题（2003003）

摘　　要：目的探讨脂蛋白（a）[Lp（a）]对大鼠肾小球系膜细胞（GMCs）增生及细胞间黏附因子-1（ICAM-1）表达的影响。方法分离Lp（a），培养GMCs，在不同时间（12、24、48、60、72h），应用不同剂量（1．25μg／L、2．5μg／L、5．0μg/L、10μg／L、20μg／L）Lp（a）处理GMCs，采用MTT法测定GMCs增生率，免疫组化法检测GMCs的增生细胞核抗原（PCNA）阳性表达率，酶联免疫法检测培养液上清的ICAM-1浓度，并与脂多糖组、对照组比较。结果与LPS组、对照组比较，随着Lp（a）剂量的增加，GMCs的MTT、PC—NA阳性表达率、上清ICAM-1浓度呈增加趋势，明显高于对照组，但低于LPS组，在Lp（a）2．5μg／L、5．0μg／L其作用达到高峰，大剂量时出现下降，剂量越大，下降越明显，Lp（a）10．0μg／L低于对照组，20．0μg／L则明显低于对照组。随着处理时间的延长，GMCs的MTT、PCNA阳性表达率、上清ICAM-1浓度呈增加趋势，但随着剂量的增加，依次于72、60、48h逐渐出现下降。GMCs上清的ICAM-1浓度与MTT和PCNA阳性表达率呈正相关。结论Lp（a）能明显影响GMCs的增生，作用呈剂量依赖性和时间依赖性，小剂量时刺激GMCs的增生，大剂量则表现为细胞毒作用。Lp（a）明显影响大鼠GMCs的ICAM-1表达，GMCs上清的ICAM-1浓度与MTT和PCNA阳性表达率呈正相关。提示Lp（a）对GMcs的作用可能与ICAM-1有关。Objective To investigate the effects of Lp（a） on proliferation GMCs of rat model induced by lipopolysaccharide and explore the possible mechanism of Lp（a） in the proliferation of rat GMCs. Methods To observe the effects of Lp （a） on proliferation of GMCs, different dosage of Lp（a） were used,. The research were divided into three groups： Control group, LPS group, Lp（a） group. After culture （at the end of 12h, 24h, 48h, 60h and 72h）, the cultured GMCs and suspension were collected to observe the rate of GMCs proliferation by MTT, the positive rate of proliferation cell nuclear antigen （PCNA） by immunohistochemisty, and the level of intercellular adhesion molecule-1 （ ICAM-1 ） by ELISA respectively. Results Compared with control and LPS group, MTT, positive rate of PCNA and ICAM-1 of GMCs were increased more significantly in Lp（a） group. MTT, the positive rate of PCNA and ICAM-1 of GMCs were increased as Lp（a） dosage increased, a maximal effect was seen when Lp（a） was 2. 5μg/L or 5.0μg/L. When the dosage continue increased, MTT, the positive rate of PCNA and ICAM-1 activity of GMCs began to decrease in Lp（a） group. ICAM-1 showed positive correlation with MTT and the positive rate of PCNA. Conclusion Lp（a） can significantly affect the rate of GMCs proliferation, and this affection is in a dosage-and time- dependent manner. Low dosage stimulates GMCs proliferation, and high dosage inhibits GMCs proliferation. ICAM-1 shows positive correlation with MTT and the positive rate of PCNA. The effect of Lp（a） on GMCs may be through ICAM-1.

关 键 词：脂蛋白(A) 肾小球膜 胞间粘附分子1 

分 类 号：R726.9[医药卫生—儿科]