酸浆宿萼总黄酮和多糖的提取及含量测定  

EXTRACTION AND DETERMINATION OF TOTAL FLAVONOIDS AND POLYSACCHARIDES FROM PERSISTENT CALYX OF PHYSALIS ALKEKENGIL. VAR. FRANCHETI(MAST.) MAKINO

作　　者：敬思群[1] 郑力[1] 苏俊[1] 

机构地区：[1]新疆大学生命科学与技术学院,新疆乌鲁木齐830046

出　　处：《食品研究与开发》2008年第6期82-84,共3页Food Research and Development

基　　金：新疆生物资源基因工程重点实验室资助(XJDX0201-2006-10)

摘　　要：用超声波提取法提取酸浆宿萼总黄酮,用乙醇回流法提取酸浆宿萼多糖。通过重现性试验和加标回收实验,验证了方法的可靠性和准确性。结果表明,新疆酸浆宿萼总黄酮的含量为1.704 mg/g,平均回收率为99.90%,RSD=1.44%(n=3);酸浆宿萼多糖的含量为0.632 g/g,平均回收率为98.92%,RSD=1.02%(n=5)。Total flavonoids was extracted by ultrasonic method and polysaccharides was extracted with ethanol by heated reflux from persistent calyx of Physalis alkekengiL. Var. Francheti （Mast.） Makino.The reliability and accuracy was proved through recurring and recovery ratio experiment.The results showed that：the content of total flavonoids was 1.704 mg/g , and the average recovery rate was 99.90 % , RSD=1.44 % （n= 3）; The content of polysaccharides was 0.632 g/g, and the average recovery rate was 98.92 % ,RSD=1.02 % （n= 5）.

关 键 词：酸浆宿萼 总黄酮 多糖 测定 

分 类 号：TQ461[化学工程—制药化工]