复方滇褐毛甘西鼠尾注射液抗大鼠在体心肌缺血-再灌注损伤作用  

The Compounded Injection Made from Sanqi and Hemaoganxishuwei Native to Yunnan,China Protecting Myocardium from Ischemia/Reperfusion Injury in Rats in Vivo

作　　者：段为钢[1] 卿晨[2] 李惠兰[1] 张荣平[3] 郑春兰[4] 

机构地区：[1]云南中医学院药理教研室,云南昆明650200 [2]昆明医学院云南省天然药物药理重点实验室 [3]昆明医学院药学院 [4]昆明医学院药理学教研室,云南昆明650031

出　　处：《昆明医学院学报》2008年第2期28-31,35,共5页Journal of Kunming Medical College

摘　　要：目的与复方丹参注射液比较,探讨复方褐毛甘西鼠尾抗大鼠在体心肌缺血再灌注损伤的作用及其在抗心肌缺血再灌注损伤方面替代丹参的可行性.方法采用在体缺血再灌注模型,缺血30 min复灌60min.实验后检测血清和灌流液中丙二醛(MDA)、乳酸脱氢酶(LDH)、肌酸激酶(CK)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)含量,并测定在体实验模型的心室梗死面积,实验过程中全程II导联记录心电图.结果(1)复方褐毛甘西鼠尾注射液能显著降低在体心脏缺血再灌注后MDA、CK、LDH水平,升高SOD和GSH-Px,相似于复方丹参;(2)褐毛甘西鼠尾复方注射液能明显减少心室梗死面积;(3)此两药均能加快缺血再灌注过程的心率,减轻ST段抬高.结论复方褐毛甘西鼠尾有较好的抗心肌缺血再灌注损伤作用,在抗心肌再灌注损伤方面可替代丹参,其机制可能与抗氧自由基损伤有关.Objective To explore the cardio protection induced by the compounded injections made from SPM ＋ PN during ischemia/reperfusion in rats in vivo, compared with injection made from SM＋PN ; And to find out whether SPM can be used as SM against cardio ischemia/perfusion injury or not. Methods We ligated rats＇ artery of left anterior descending （LAD） for 30 min, then let it loose for 60 min, We examined maleic dialdehyde （MDA）, lactate dehydrogenase （LDH）, creatine kinase （CK）, superoxide dismutase （SOD）, and glutathione peroxidase （GSH-Px） in rats＇ serum after reperfusion, and checked ventrical ischemical area. During experiment, we also recorded elctrocardiogram （ECG） to check heart rate and ST-segment. Results （1） Injection ofSPM ＋ PN was able to decrease MDA, CK, LDH, and increase SOD, GSH-Px, in vivo, and not less powerful than that of SM＋PN; （2） The injection of SPM＋PN alleviated ischemia area, not less powerful than that of SM＋PN also; （3） Two injections was able to quicken heart rate and alleviate ST-segment. Conclusions The compounded injection from SPM＋PN employ cardio protection against ischemia/perfusion injury, which is not less powerful than the injection from SM＋PN, and which suggest that SPM can be a likely replacer for SM to protect myocardium from ischemia/reperfusion injury. The mechanisms of which may be associated with deactivating oxygen free radicles.

关 键 词：缺血再灌注损伤 保护作用 褐毛甘西鼠尾 三七 

分 类 号：R285.5[医药卫生—中药学]