新型复合脂质体Cerasome稳定性的研究  

Morphological Stability of a Novel Organic-Inorganic Hybrid Vesicle "Cerasome"

作　　者：张乾顺[1] 田文杰[2] 金黎明[2] 刘业伟[2] 菊池纯一 靳学慧[1] 

机构地区：[1]黑龙江八一农垦大学植物科技学院,黑龙江大庆163319 [2]大连民族学院生命科学学院,辽宁大连116605 [3]奈良先端科学技术大学院大学物质创成科学研究科,日本国奈良6300192

出　　处：《大连民族学院学报》2008年第3期214-216,共3页Journal of Dalian Nationalities University

基　　金：国家自然科学基金资助项目(20502003;20710102004);国家外国专家局资助项目;辽宁省高校科研计划资助项目(20060153)

摘　　要：合成了复合脂质N,N-二-十六烷基-Nα-6-(3-三乙氧硅烷基)丙基二甲胺基己酰基-L-丙氨酰胺(1),其结构经薄层色谱(TLC)、1H核磁共振(NMR)及元素分析表征得以证实。将该脂质分散到水溶液中并进行超声,其头部将水解进而缩合形成表面具有陶瓷或硅覆盖的脂质体,因此将其命名为Cerasome。用十六烷基三甲基溴化铵(CTAB)作为添加的表面活性剂,采用荧光分光光度计通过测定脂质体的光散射强度,对Cerasome及作为对照的合成肽脂N,N-二-十六烷基-Nα-6-三甲胺基己酰基-L-丙氨酰胺(2)和磷脂酰胆碱(3)脂质体的稳定性进行了评价,结果Cerasome表现出了异常高的稳定性。An organic - inorganic hybrid lipid N, N - dihexadecyl - N^α - [ 6 - [ （3 - triethoxysilyl） propyldi -methylammonio ] hexanoyl I alaninamide bromide was synthesized. The compound was characterized by thin layer chromatography,^1 HNMR spectra and elemental analysis. The lipid has a （triethoxysilyl）propyl group as an inorganic precursor on the quarternized ammonium nitrogen. Upon sonication of the lipid dispersed in aqueous HEPES buffer, it self- assembles with concomitant hydrolysis of the inorganic precursor moiety followed by condensation to give partially ceramic - or silica -coated liposome （Cerasome）. Morphological stability of the Cerasome was evaluated by light scattering intensity employing a fluorescence spectrophotometer with the addition of the surfactant CTAB. For comparison, the morphological stability of liposomes derived from a synthetic peptide lipid N, N - dihexadecyl - N^α - [ 6 - （ trimethylam- monio） hexanoyl ] alanin - amide bromide （2） and a phospholipid dimyristoylphosphatidylcholine （3） were examined under the similar sonication conditions. The results indicate that the Cerasome is highly stable as compared to the conventional lioosornes.

关 键 词：有机-无机复合体 Cerasome 脂质体稳定性 

分 类 号：O062[理学]