种番鸭减蛋综合征病毒六邻体蛋白基因的克隆与序列分析  

Cloning and sequence analysis of hexon protein gene of egg drop syndrome virus isolated from breeding muscovy duck

作　　者：陈红梅[1] 傅光华[1] 程龙飞[1] 施少华[1] 彭春香[1] 黄瑜[1] 

机构地区：[1]福建省农业科学院畜牧兽医研究所,福建福州350013

出　　处：《福建农林大学学报（自然科学版）》2008年第3期303-306,共4页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建省农业科学院青年科技人才创新基金资助项目

摘　　要：运用PCR技术从表现产蛋异常的种番鸭分离鉴定的减蛋综合征病毒(EDSV)E05株中分段扩增六邻体蛋白基因,分别与克隆载体pMD18-T连接后,转化感受态细胞,对经PCR鉴定和酶切鉴定均为阳性的克隆进行测序.结果表明,E05株六邻体蛋白基因长度为2733 bp,共编码910个氨基酸.序列分析表明,该株病毒与鸡源EDSV 127株和鹌鹑源EDSV QU株六邻体蛋白基因的核苷酸同源性分别为99.6%和96.0%,氨基酸同源性分别为99.9%和99.3%.Hexon protein gene of egg drop syndrome virus （ EDSV）, named strain E05 which was isolated from breeding muscovy duck with irregular egg dropping was amplified by PCR and cloned into pMD18-T vector. The recombinant plasmids were identified by digested restriction endonuclease and PCR. The result showed that the nucleotide length of hexon protein gene encoding 910 amino acids was 2733 bp. Compared with the 127 strain chicken-origin EDSV and QU strain quail-origin EDSV, the nucleotide sequence homologies of hexon protein gene were 99.6% and 96.0%, the amino acid sequence homologies were 99.9% and 99.3%, respectively.

关 键 词：种番鸭 减蛋综合征病毒(EDSV) 六邻体蛋白基因 克隆 序列分析 

分 类 号：S834.89[农业科学—畜牧学] S852.659.1[农业科学—畜牧兽医]