银杏叶提取物对哮喘大鼠气道嗜酸性粒细胞浸润的影响  

The Effect of Ginkgo Biloba on the Infiltration of Eosinophil in Airway of Asthma Rats

作　　者：吴美玲[1] 林益平[1] 金志华[1] 魏艳丽[1] 林兴秋[1] 

机构地区：[1]金华职业技术学院,浙江金华321007

出　　处：《金华职业技术学院学报》2008年第2期9-13,共5页Journal of Jinhua Polytechnic

基　　金：2006年度校级省自然基金预研项目(2006Z005)

摘　　要：目的:观察银杏叶提取物(Egb)对哮喘大鼠气道嗜酸性粒细胞趋化因子(Eotaxin)的表达及嗜酸性粒细胞(EOS)在气道局部浸润的影响。方法:复制哮喘大鼠模型,用免疫组化检测肺组织NF-κBp65亚基及Eotaxin蛋白活性;细胞分类计数法检测支气管肺泡灌洗液(BALF)中EOS的绝对计数和百分比;病理学方法观察气道炎症程度。结果:哮喘组肺组织NF-κBp65(0.256±0.063)及Eotaxin(0.197±0.055)表达量均显著高于正常组(分别为0.015±0.006,0.034±0.008)(均为P<0.01)。Egb组肺组织NF-κBp65(0.104±0.056)及Eotaxin(0.115±0.010)的表达均显著低于哮喘组(均为P<0.01);Egb组BALF中EOS的绝对计数和百分比均低于哮喘组(均为P<0.01);光镜下观察Egb组气道炎症较哮喘组显著减轻。结论:哮喘组肺组织NF-κBp65及Eotaxin表达显著增强,Egb能有效抑制哮喘大鼠肺组织NF-κBp65的活性从而抑制Eotaxin的转录合成,减少EOS在局部的浸润,减轻气道炎症。Objective： To study the role of Extract of ginkgo biloba（Egb） in regulating the activity of NF-κBp65 and Eotaxin protein of asthma rats,and in effecting the infiltration of eosinophil within airway.Method： Thirty-six Sprague-Dawley（SD） rats were divided into three groups of 12 each.The asthmatic model was established by ovalbumin（OVA） injection and inhalation.Group E were pretreated with Egb injection with 8mg·kg^-1·d^-1 for 14 days.The protein NF-κBp65 and Eotaxin in lung tissue were assessed by using immunohistochemisty.The numbers of EOS and the EOS% in BLAF were counted by different count fluids.The condition of airway inflammation were observed by pathology.Results： Twenty-four hours after the last antigen challenge,the protein expression of NF-κBp65（0.256±0.063）and Eotaxin（0.197±0.055） in group A were significantly increased compared with group C（0.034±0.008, 0.010±0.005 p〈0.01,respectively）.The protein expression of NF-κBp65（0.062±0.014）and Eotaxin（0.115±0.010）in group E were significantly decreased compared with group A（p〈0.01,respectively）.The numbers of EOS and the EOS% in BLAF of group E were significantly decreased compared with group A（p〈0.01,respectively）.The conditions of airway inflammation of group E were significantly decreased compared with group A under light microscope.Conclusion： In asthmatic rats,the protein of NF-κBp65 and Eotaxin were increased significantly,Egb could inhibit the expression of the protein of NF-κBp65 and hold down the transcription of Eotaxin,and then reduce the infiltration of eosinophil within airway and decrease the condition of airway inflammation.

关 键 词：哮喘 大鼠 核转录因子 嗜酸性粒细胞趋化因子 嗜酸性粒细胞 银杏叶提取物 

分 类 号：R562.25[医药卫生—呼吸系统]