电针任督脉经穴对脑缺血大鼠侧脑室下区ERK通路的影响  

The Role of Electroacupuncture on Extracellular Signal-regulated Kinase Pathway in SVZ of Focal Cerebral Ischemia

作　　者：罗文舒[1] 杨卓欣[1] 于海波[1] 皮敏[1] 刁利红 

机构地区：[1]深圳市中医院,广东深圳518033 [2]蛇口人民医院,广东深圳518000

出　　处：《中医药导报》2008年第5期3-5,共3页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金资助课题(NO.30371808)

摘　　要：目的:研究电针任督脉经穴对局灶性脑缺血侧脑室下区(SVZ)细胞外信号调节激酶(ERK)通路的调节作用。方法:线栓法制作大鼠大脑中动脉栓塞(MCAO)模型。通过Westem blot方法研究各组ERK1/2磷酸化的活性特征。结果:电针大鼠任督脉经穴治疗后ERK1/2磷酸化水平在MCAO 7 d后开始提高,这一促进作用至少在MCAO 14 d后要强于单独电针督脉治疗。结论:电针任督脉经穴对大鼠局灶性脑缺血的ERK通路起着重要的调节作用。Objective： To determine the role of electroacupuncturing Ren and Du vessels on extracellular signal-regulated kinase pathway in SVZ of focal cerebral ischemia. Methods： Male adult Wistar rats underwent right middle cerebral artery occlusion （MCAO）after introducing a nylon suture to the right internal carotid artery. ERK1/2 phosphorylation were detected by Western blot analysis. Results： In electroacupuncturing Ren and Du vessels treated rats,7 d after MCAO,the level of pERK1/2 rised compared with the control group rats;and 14 d after MCAO,the level of pERK1/2 did compared with electroacupuncturing Du vessel treated rats. Conclusions： This study demonstrates electroacupuncturing Ren and Du vessels plays an important role on extracellualar signal-regulated kinase pathway in focal cerebral ischemia.

关 键 词：脑 缺血 电针 丝裂原活化蛋白激酶 大脑中动脉梗死 大鼠 

分 类 号：R245.91[医药卫生—针灸推拿学]