纳米羟基磷灰石胶体的制备及工艺特征  

Techniques for the preparation of nano-size hydroxyapatite

作　　者：王瑞芳[1] 钟银屏[1] 熊成东 何成生[1] 樊翠蓉[1] 谢兴益[1] 

机构地区：[1]四川大学高分子科学与工程学院,四川省成都市610065 [2]迪康中科生物医学材料有限公司,四川省成都市610065

出　　处：《中国组织工程研究与临床康复》2008年第14期2679-2682,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:各种形式的羟基磷灰石如粉末、薄膜和复合物等在医学应用方面得到广泛的关注,但羟基磷灰石制备过程中粒子易团聚的问题还没有彻底解决。目的:实验拟制备透明的、稳定存在的纳米羟基磷灰石水溶胶,并探索羟基磷灰石胶体的制备工艺条件。设计、时间及地点:分组对比观察实验,于2007-03/06在四川大学高分子科学与工程学院生物材料实验室及迪康中科生物医学材料有限公司完成。材料:磷酸铵[(NH4)3PO4·3H2O],分析纯,广东汕头市西陇化工厂产品;硝酸钙[Ca(NO3)2·4H2O],分析纯,天津市东升化学试剂厂产品;柠檬酸三钠(C6H5O7Na3·2H2O),分析纯,成都科龙化工试剂厂。方法:在传统的湿化学方法即用硝酸钙、磷酸铵和氨水合成羟基磷灰石的基础上,引入柠檬酸钠作为分散剂,制备纳米羟基磷灰石水溶胶。主要观察指标:磷酸铵滴加时间、磷酸铵滴加温度和柠檬酸钠用量对羟基磷灰石粒径的影响。结果:①合成产物的谱图中所有的衍射峰与羟基磷灰石标准衍射图的峰位基本相同,没有加分散剂的对照样峰形更加完整,而采用柠檬酸钠作分散剂得到的羟基磷灰石的衍射峰相对较弱,结晶不完全。②在保持其他合成条件不变的情况下,随着磷酸铵溶液滴加温度的增加,所得羟基磷灰石胶体的最小粒径逐渐增大。在其他实验条件不变的情况下,当分散剂浓度低的时候,得到的羟基磷灰石粒径较大;当分散剂浓度过高的时候,得到的羟基磷灰石粒径也较大。结论:用柠檬酸钠制备羟基磷灰石胶体的适宜条件:磷酸铵滴加速度7.8mL/min,滴加温度15℃,柠檬酸钠浓度0.227mol/L,氨水浓度0.066mol/L。BACKGROUND： Hydroxyapatite has been studied extensively for medical applications in varied forms, such as powders, films and composites, but particle aggregation is still a problem of the preparation of hydroxyapatite. OBJECTIVE： To prepare transparent and stable nano-size hydroxyapatite gel, and search out the suitable preparation techniques of hydroxyapatite gel. DESIGN, TIME AND SETTING： The grouping controlled observation experiment was carried out in the Laboratory of Biomaterials, Department of Polymer Science and Engineering of Sichuan University and Dikang Biomedical Co., Ltd from March to June in 2007. MATERIALS： Ammonium phosphate[（NH4）3PO4 3H2O], analytical pure, was the product of Shantou Xilong Chemical Industry Factory Co., Ltd.; calcium nitrate[Ca（NO3）2 4H2O], analytical pure, was the product of Tianjin Chemical Reagent Co., Inc; sodium citrate （C6H5O7Na3. 2H2O）, analytical pure, was the product of Chengdu Kelong Chemical Reagent Factory. METHODS： Based on the traditional wet chemical method, hydroxyapatite was synthesized with calcium nitrate, ammonium phosphate and ammonia water, taking sodium citrate as a dispersant. MAIN OUTCOME MEASURES： Changes of hydroxyapatite particle size influenced by ammonium phosphate dropping time, ammonium phosphate dropping temperature and the amount of sodium citrate. RESULTS： All the position of diffraction peaks in synthesized product spectrogram were identical with those in hydroxyapatite standard diffractogram, and the peak of controls without dispersing agent was more intact. While the diffraction peak was relatively weak in hydroxyapatite that synthesized with sodium citrate as dispersant, and crystallization was not complete.ln the synthesis process, with the other conditions being fixed, the minimal particle size of hydroxyapatite increased gradually with the increasing dropping temperature of ammonium phosphate. When the other conditions were unchanged, the particle size of synthesized hydroxyapatite was large when the dispersant

关 键 词：羟基磷灰石 柠檬酸钠 胶体 制备 

分 类 号：R318[医药卫生—生物医学工程]