TSA对甲状腺鳞癌细胞株SW579增殖的影响  

Effects of TSA Acid on Proliferation in Thyroid Squamous Cell Carcinoma Cell Line SW579

作　　者：王新[1] 肖建英[1] 张秀梅[1] 李男[1] 宋其蔓[1] 

机构地区：[1]辽宁医学院生物化学教研室,辽宁锦州121001

出　　处：《辽宁医学院学报》2008年第1期16-19,共4页Journal of Liaoning Medical University (LNMU) Bimonthly

基　　金：辽宁省教育厅资助项目(编号:05L132)

摘　　要：目的探讨曲古押菌素A(trichostatin A,TSA)对甲状腺鳞癌细胞株SW579生长增殖的影响。方法分别以终浓度为25、50、100、200、400 nmoL/L的TSA作用于SW579细胞株,在倒置显微镜下观察细胞生长情况及形态变化;采用四甲基偶氮唑蓝比色法(MTT)检测细胞的体外增殖、吖啶橙染色荧光显微镜观察细胞形态及细胞凋亡、流式细胞术检测细胞凋亡率的改变。结果TSA作用后对细胞的增殖有抑制作用;吖啶橙染色后光镜下观察发现有典型的凋亡小体生成;流式细胞仪分析凋亡率随TSA浓度的升高而升高(P<0.05)。结论不同浓度的TSA可抑制SW579细胞增殖,促进细胞凋亡,且呈剂量依赖性。Objective To study the effects of trichostatin A （TSA） on apoptosis and proliferation of thyroid squamous cell carcinoma SW579. Methods Treated with different doses of TSA （final concentration of 25 nmol/L, 50 nmol/L, 100 nmol/L, 200 nmol/L, 400nmol/L）, the cells were observed with an inverted microscope; The proliferation activities of cell line were determined by using methyl thiazolyl tetrazolium （MTT） assay, Cell morphologies and apoptotic were observed by fluorescence microscope stained with acridine orange , Flow cytometry was adopted to examine apoptotic rate. Results TSA inhibited significantly the growth of cell line and the effect was with a dose dependent manner. Typical apoptotic morphologic feature was discovered under fluorescence micro- scope. The apoptotic peak was detected with FCM and apoptotic rate increased with the concentration raised, Conclusions TSA can inhibit the proliferation and induce apoptosis in the thyroid cancer cells with a dose - dependent manner.

关 键 词：曲古抑菌素A 甲状腺鳞癌 细胞增殖 细胞凋亡 

分 类 号：R979.1[医药卫生—药品]