外源过表达CPNE5基因增强HEK293细胞对Zeocin的敏感性  

Overexpression of CPNE5 Gene Can Enhance Zeocin-Sensitivity of HEK293 Cells

作　　者：王晓文[1] 吴彦瑞[1] 吴燕[1] 靳雁斌[1] 刘淑红[1] 范文红[1] 范明[1] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850

出　　处：《生物技术通讯》2008年第3期336-339,共4页Letters in Biotechnology

基　　金：国家高技术研究发展计划项目(2002BA711A01-03)

摘　　要：目的:探讨Zeocin处理是否能增强CPNE5基因诱导细胞凋亡的作用。方法:分别构建CPNE5基因稳定过表达和抑表达载体,转染HEK293细胞,形成克隆后,用RT-PCR、MTT法分析CPNE5基因过表达和抑表达的HEK293单克隆细胞对Zeocin作用的敏感性。结果:CPNE5基因过表达的HEK293单克隆细胞对Zeocin反应敏感性增加。结论:Zeocin能增强CPNE5基因诱导细胞凋亡的作用。Objective： To investigate the effect of Zeocin-treatment on CPNE5 gene induced cell apoptosis. Methods： The overexpression and suppression vector of CPNE5 gene were constructed and transfected into HEK293 cells. After clone formation assays, CPNE5 gene overexpression and suppression monoclones were separately analyzed using RT-PCR, MTT assay, and Zeocin-sensitivity assay. Results： Overexpression of CPNE5 gene monoclone cells were more sensitive to Zeocin treatment. Conclusion： Zeocin can enhance the effect of CPNE5 gene induced cell apoptosis in HEK293 cells.

关 键 词：CPNE5基因 过表达 抑表达 Zeocin敏感性 

分 类 号：Q25[生物学—细胞生物学]