烹调油烟挥发性有机物对人胚肺二倍体成纤维细胞活性氧水平的影响  

The Alteration of Reactive Oxygen Species Level in the Human Embryonic Diploid Lung Fibroblasts Induced by Cooking Oil Fume Volatile Organic Compounds

作　　者：于仲波[1] 吴南翔[1] 陈琼姜[1] 谭玉凤[1] 金锋[1] 王怡亭[1] 陶核[1] 金勇[1] 柴剑荣[1] 

机构地区：[1]浙江省医学科学院卫生学研究所,浙江杭州310013

出　　处：《生物技术通讯》2008年第3期398-400,共3页Letters in Biotechnology

基　　金：浙江省卫生厅资助项目(2002ZD002)

摘　　要：目的:研究烹调油烟挥发性有机物(COF VOCs)对人胚肺二倍体成纤维细胞(HELF)活性氧(ROS)水平的影响。方法:用活性炭采集烹调油烟挥发性有机物,对HELF进行染毒,采用MTT试验测得半数抑制浓度(IC50),设计剂量和暴露时间,设立添加和不添加维生素C组,以2',7'-二氯双氢荧光素二乙酸酯(DCFH-DA)和双氢罗丹明123(DHR123)进行标记,通过流式细胞术检测细胞ROS水平。结果:COFVOCs刺激HELF12、24、48h的IC50分别是104.9、111.9和127.2μg/mL;HELF胞质内ROS平均荧光强度随COFVOCs剂量增加而增高;线粒体内ROS24、48h暴露于0.8、4μg/mL剂量与阴性对照组相比有统计学差异;维生素C预处理后,ROS平均荧光强度较未预处理时均有所降低,100μg/mL剂量组前后差异有统计学意义。结论:COFVOCs可引起HELF内ROS水平升高,维生素C对细胞的ROS升高有一定的抑制作用。Objective： Reactive oxygen species（ROS） induced by cooking oil fume volatile organic compounds（COF VOCs） was studied on human embryonic diploid lung fibroblasts（HELF）. Methods： Glass fibre filter film was used to collect COF VOCs. IC50 was determined by MTT assay. HELF cells were exposed to COF VOCs with or without vitamin C respectively. Then ROS analysis with 2′,7′-dichlorofluoresein diacetate（DCFH-DA） and dihydrorhodamine 123（DHR123） was performed. Results： The IC50 for 12, 24, 48 hours was 104.9, 111.9 and 127.2 μg/mL respectively. The mean of fluorescence intensity increased as the dose increased in plasma. Significant difference was observed between 0.8 and 4 μg/mL dose group and negative control group for 24, 48 hours exposure duration in mitochondria. With vitamin C, the mean decreased com- paring with the group without vitamin C, significant difference was observed at 100 μg/mL dose group. Conclusion： In the range of experimental dose and exposure duration, ROS increase in both plasma and mitochondria. Vitamin C could decrease the level of ROS.

关 键 词：烹调油烟挥发性有机物 活性氧 维生素C 人胚肺二倍体成纤维细胞 

分 类 号：R114[医药卫生—卫生毒理学]