赤芍对球囊损伤术后血管内膜单核细胞趋化蛋白-1基因表达的影响  

Effect of Chishao (赤芍) on monocyte chemotactic protein-1 mRNA expression of vascular intima after balloon injury in cholesterol-fed rabbits

作　　者：朱慧民[1] 牟华明[2] 

机构地区：[1]浙江省台州市中心医院老年医学科、浙江省台州市老年医学研究所、浙江省医学重点扶持学科老年医学重点实验室,浙江台州 318000 [2]重庆三峡中心医院心内科,重庆 404000

出　　处：《中国中西医结合急救杂志》2008年第3期138-141,F0003,共5页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：浙江省卫生厅科学研究基金项目(2004B204);浙江省台州市科技局医药卫生科技计划项目(043236)

摘　　要：目的观察单核细胞趋化蛋白-1(MCP-1)在高脂喂养兔颈动脉球囊损伤术后内膜增生中的机制,以及赤芍的干预作用。方法将40只新西兰大白兔随机分为对照组、模型组及赤芍高、中、低剂量组。对照组兔喂饲普通饲料;其余各组喂饲高脂饲料(93%普通饲料+2%胆固醇+5%猪油);赤芍组按赤芍提取物不同剂量给药:高剂量组相当于生药3g·kg-1·d-1,中剂量组相当于生药2g·kg-1·d-1,低剂量组相当于生药1g·kg-1·d-1。各组动物喂饲8周后行颈动脉球囊损伤术制备颈总动脉内膜再狭窄模型。于10周末取各组兔耳静脉血,测定血清总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、载脂蛋白B(Apo-B)水平;取血后活杀动物,取损伤血管做冰冻切片,观察血管内膜增生情况,用原位杂交、免疫组化分析及逆转录-聚合酶链反应(RT-PCR)检测MCP-1 mRNA表达。结果与模型组比较,赤芍各剂量组TC、TG、LDL-C、Apo-B均明显降低,HDL-C则明显升高(P<0.05或P<0.01);内膜增生面积、内膜增生/中膜面积比值显著减小,MCP-1mRNA表达均减少,其中以赤芍高、中剂量组最为显著(P<0.05或P<0.01)。结论赤芍可减轻兔颈动脉球囊损伤术后内膜增生程度,抑制血管壁MCP-1 mRNA表达。Objective To observe the mechanism of monocyte chemotactic protein-1 （MCP-1） mRNA expression on neointimal hyperplasia after carotid artery balloon injury in cholesterol-fed rabbits, and the effect of Chishao （赤芍, Radix Paeoniae Rubra, a traditional Chinese herbs） on it. Methods The rabbits （n=40） were randomly divided into control group, model or cholesterol group and Chishao high, middle, and low dose groups. The normal control group was fed with ordinary fodder （n=8）; other rabbits were all fed with general fodder including 2% cholesterol and 5% lard; in the 3 Chishao groups, Chishao extract was added to the cholesterol-fed rabbits [The dose of Chishao extract equivalent to its raw herb 3 g·kg^-1·d^-1 in the high dose （n=8）, 2g·kg^-1·d ^-1 in the middle dose （n=8）, and lg·kg^-1·d^-l in the low dose group （n= 8）]. After feeding for 8 weeks, balloon injury was performed in rabbits with common carotid artery atherosclerotic constriction induced by high cholesterol diet. Ten weeks after the operation, venous blood was collected from the ear vein to measure the levels of total cholesterol （TC）, triglycerides （TG）, low density lipoprotein-cholesterol （LDL-C）, high density lipoprotein-cholesterol （HDL-C） and apo-lipoprotein B （Apo-B） ; after collection of blood, the rabbits were sacrificed, and the injured blood vessels were frozen and sectioned for the morphometrical investigation of intimal hyperplasia. MCP-1 mRNA expression was determined by in situ hybridization, immunohistochemistry and reverse transcription-polymerase chain reaction （RT-PCR）. Results Compared with the cholesterol group, TC, TG, LDL-C and Apo-B were markedly reduced, while HDL-C were markedly increased in Chishao high, middle and low-dose groups （P〈 0. 05 or P〈0.01）; the area of intimal hyperplasia and the ratio of intimal hyperplastic area layer area were significantly decreased（P〈0.05 or P〈0.01）. MCP-1 mRNA expression was decreased in all the groups, and in Chishao h

关 键 词：赤芍 颈动脉球囊损伤术 血管内膜增生 单核细胞趋化蛋白-1 

分 类 号：R285[医药卫生—中药学]