热作用致心肌组织在可见与近红外范围内的光学特性变化  

Changes in Optical Properties of in vitro Myocardium Tissue Due to Heating in the Visible and Near-Infrared Range

作　　者：敖荟兰[1] 邢达[1] 魏华江[1] 巫国勇[2] 鲁建军[2] 

机构地区：[1]华南师范大学激光生命科学研究所激光生命科学教育部重点实验室,广东广州510631 [2]中山大学第一附属医院心胸外科,广东广州510080

出　　处：《中国激光》2008年第5期792-796,共5页Chinese Journal of Lasers

基　　金：国家自然科学基金(30470494，30627003，60678050);广东省自然科学基金(015012)资助项目

摘　　要：研究了热作用对心肌组织在可见及近红外光谱范围内的光学特性改变。利用带积分球附件的分光光度计测量了心肌组织样品的漫反射率和总透射率,再用反向倍增(IAD)法从这两测量值中获取组织的吸收系数、约化散射系数和光学穿透深度。结果表明,自然的及热处理后的心肌组织的吸收系数、约化散射系数和光学穿透深度都是随着辐照波长的变化而显著变化的。自然的及热凝固后(80℃热处理)的心肌组织的吸收系数均在550 nm附近有一正峰,峰值分别为0.74 mm^(-1)和1.16 mm^(-1)。自然的心肌组织的约化散射系数在550 nm附近出现一正峰,峰值为0.25 mm^(-1);高温处理后此峰消失,而在590～625 nm出现上升坡。低温热处理对心肌组织的吸收系数没有明显的影响,高温热处理后心肌组织的吸收系数增大;心肌组织的约化散射系数随热处理的温度上升而显著地升高;心肌组织的光学穿透深度随热处理的温度上升而显著地减小。The effects of optical properties of myocardium tissue due to heating in the visible and near-infrared spectral range were investigated. A spectrophotometer was used to measure the diffuse reflectance and total transmittance of the tissue samples, and the inverse adding-doubling （IAD） method was applied to assess the absorption coefficients, the reduced scattering coefficients and the optical penetration depth of tissue from the measurements. The results of measurement show that the absorption and reduced scattering coefficients and optical penetration depths of myocardium tissues vary with the change of irradiation wavelength. There are a positive peaks at 550 nm for the absorption coefficients of both native and thermocoagulated （treated with 80 ℃ ） myocardium, and the peak values for native and thermocoagulated myocardium are 0.74 mm^-1 and 1.16 mm^-1 , respectively. There is a positive peak at 550 nm for the reduced scattering coefficients of native myocardium and the peak value is 0.25 mm ^-1 , whereas the peak disappears after myocardium tissue treated with high temperature. Further more a new increase appears at the wavelength range of 590-625 nm. The absorption coefficient of myocardium tissue is not significantly changed by treating with lower temperature but increases slightly with higher temperature. The reduced scattering coefficient of myocardium tissue is significantly increasing with the increase of treatingtemperature. And the optical penetration depth of myocardium tissue is significantly decreasing with the descrease of treating temperature.

关 键 词：医用光学与生物技术 组织光学 积分球 光学特性 心肌组织 热作用 反向倍增法 

分 类 号：R318.51[医药卫生—生物医学工程]