小麦转录因子TaDREB6基因的克隆及鉴定  

Isolation and Characterization of a Transcription Factor TaDREB6 Gene from Triticum aestivum L.

作　　者：倪志勇[1,2] 徐兆师[2] 刘丽[2] 李连城[2] 柴岩[1] 陈明[2] 马有志[2] 

机构地区：[1]西北农林科技大学农学院,陕西杨陵712100 [2]国家农作物基因资源与基因改良重大科学工程,农业部作物遗传育种重点开放实验室,中国农业科学院作物科学研究所,北京100081

出　　处：《麦类作物学报》2008年第3期357-363,共7页Journal of Triticeae Crops

基　　金：国家高技术研究发展计划(“863”计划)项目(2007AA10Z130);国家自然科学基金项目(30700504);北京市科技计划项目小麦转基因育种(Z07070501770702)

摘　　要：为了克隆小麦干旱应答基因,构建了干旱诱导的小麦cDNA文库,并从文库中分离了一个DREB类转录因子基因TaDREB6。序列分析表明,TaDREB6具有一个837 bp的开放阅读框和242 bp的3'非编码区,推测的氨基酸序列中含有一个高度保守的AP2/EREBP结构域。采用该基因特异引物PCR技术对一套中国春缺体-四体材料进行扩增,将TaDREB6定位于3A染色体上,这是首次将一个小麦DREB基因定位在特定的染色体上。RT-PCR分析表明,TaDREB6基因受干旱胁迫诱导表达;亚细胞定位结果表明,TaDREB6-hGFP融合蛋白定位于细胞核中。上述结果说明,小麦TaDREB6基因编码的蛋白可能在细胞核内对干旱胁迫应答反应起调控作用。In search for genes response to drought stress in wheat （Triticum aestivum L. ）, a cDNA library was constructed from drought-treated wheat seedlings, and a DREB-like transcription factor gene, TaDREB6 was isolated. TaDREB6 had an open reading frame of 837 bp with a 3＇-UTR of 242 bp, with a highly conserved AP2/EREBP domain in the encoded putative protein. Using a nullisomic-tetrasomic series of T. aestivum L. cv. Chinese Spring, the TaDREB6 gene was located on chromo-some 3A. The result of RT-RCR indicated that TaDREB6 gene was obviously induced by drought stress, suggesting an important role of TaDREB6 in response to drought stress in wheat. Subcellular localization assay indicated the TaDREB6-hGFP fusion protein was targeted in the nucleus. Above results suggested that TaDREB6 transcription factor might be involved in drought stress response and functioned in the nucleus.

关 键 词：小麦 干旱 DREB 基因克隆 

分 类 号：S512.1[农业科学—作物学] S336