优质高分子量麦谷蛋白亚基1Dx5+1Dy10的分子检测  

Molecular Detection of High Molecular Weight Glutenin Subunits 1Dx5+1Dy10 Related to Wheat Bread Quality

作　　者：刘东涛[1] 陈荣振[1] 刘世来[1] 王来花[1] 李德民[1] 张会云[1] 王静[1] 冯国华[1] 

机构地区：[1]江苏徐淮地区徐州农业科学研究所,江苏徐州221121

出　　处：《麦类作物学报》2008年第3期425-429,共5页Journal of Triticeae Crops

基　　金：国家863计划项目(2006AA100102);国家科技支撑计划项目(2006BAD01A02-18);国家农业科技成果转化项目(2006GB2C100103);江苏省高技术研究项目(BG2006305)

摘　　要：为给优质强筋小麦的选育提供高效、快速、稳定的选择手段,以已知高分子量麦谷蛋白亚基组成的10个小麦品种为材料,比较了5个已报道的高分子量麦谷蛋白亚基1Dx5(1Dy10)的PCR分子标记,并对12个DNA模板进行了分子检测。结果表明,在5个分子标记中,有1个分子标记是非特异的,有2个分子标记是显性的特异标记,另2个则是共显性的分子标记。其中,由引物Dx-F、Dx5-F和Dx-R组成的共显性标记,能稳定地扩增出5亚基和非5亚基的特异序列,并且无非特异PCR产物,是用于高分子量麦谷蛋白亚基1Dx5分子检测的最理想标记。The high molecular weight （HMW） gluentin subunits 1Dx5＋1Dy10, encoded byGlu-D1 , are firmly associated with good bread-making quality. In order to provide a quick, stable and efficient means for the selection of gluentin subunits 1Dx5＋1Dy10, five molecular PCR markers of 1Dx5 （1Dy10） were compared in this work. Ten wheat varieties were used, of which HMW glutenin sub- units compositions have been known. The DNA of two couple of varieties, with different HMW glute-nin alleles in 1D locus, was mixed respectively to make two heterogonous DNA samples artificially. PCR assay were done with 12 DNA samples. The results showed there were 2 specific dominant PCR markers for 1Dx5 subunit, and 2 specific co-dominant markers for 1Dx5 and 1Dy10, respectively. And the fifth marker had no different amplicon between 1Dx5 and non-lDx5 subunits. The third marker, composed of three primers Dx-F, DxS-F and Dx-R, was a specific co-dominant PCR marker for 1Dx5 subunit, and had no non-specific amplicon. It was strongly recommended to be used in wheat molecu- lar assistant selection （MAS） breeding for good bread-making quality in China.

关 键 词：小麦 高分子量麦谷蛋白亚基(HMW-GS) PCR 分子标记 

分 类 号：S512.1[农业科学—作物学] S331