金龟子绿僵菌菌株培养基的改良  

Improvement in Cultural Medium for Metarhizium anisopliae Strains MA4 and MAlm1

作　　者：杨腊英[1] 石晓珍[1] 刘丽[2] 甘露[2] 文明富[2] 黄俊生[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海南儋州571737 [2]海南大学农学院,海南儋州571737

出　　处：《热带作物学报》2008年第2期210-214,共5页Chinese Journal of Tropical Crops

基　　金：国家科技基础条件平台建设资助项目(2005DKA21201-18);中央级公益性科研院所基本科研业务费专项(2007HZS1J007);国家科技支撑计划项目(2007BAD48B02)

摘　　要：采用液体振荡与固体平板双培养的方法,对金龟子绿僵菌MA4,MAlm1两菌株的培养基进行改良。研究结果表明:MA4,MAlm1菌株的最适复合碳源均为玉米粉、最适氮源为蛋白胨、最适碳氮比为4:1(m:m,下同)、碳浓度分别为0.5,1.0mol/L,即改PPDA培养基为:MA4菌株以11.8g/L的玉米粉代替蔗糖,17.1g/L的酵母浸膏代替蛋白胨;而MAlm1菌株的则以23.6g/L的玉米粉代替蔗糖,蛋白胨由10.0g/L更换为16.2g/L。Metarhizium anisopliae is a broad spectrum entomopathogenic fungus. It is significant to increase the growth rate and sporulation of this fungus. So an attempt was made to conduct submerged shaking culture and solid plate culture to improve the PPDA medium. For the MA4 and MAlm1 strains, the optimal carbon sources were found to be both corn powder, the optimal nitrogen source be yeast soaking flour and peptone respectively, the optimal carbon nitrogen ratio be both 4：1, and the optimal carbon density be 0.5 mol/L and 1.0 mol/L respectively. So for the improved PPDA medium 11.8 g/L corn powder and 17.1 g/L yeast soaking flour were used in place of sucrose and peptone for the culture of MA4 strain; 23.6 g/L corn powder was used to replace sucrose for the culture of MAlm1 strain, and the peptone was changed from 10.0 g/L to 16.2 g/L.

关 键 词：金龟子绿僵菌 菌丝体干重 次代产孢量 培养基配方优化 

分 类 号：Q949.32[生物学—植物学]