橡胶树白粉病菌rDNA-ITS序列及其系统发育分析  

Phylogenetic Relationships and rDNA-ITS Sequence Analyses of Oidium heveae,a Powdery Mildew of Rubber Tree

作　　者：刘先宝[1] 高宏华[1] 蔡吉苗[1] 潘羡心[1] 张科立[1] 黄贵修[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所海南省热带农业有害生物检测监控重点实验室,海南儋州571737

出　　处：《热带作物学报》2008年第2期215-219,共5页Chinese Journal of Tropical Crops

基　　金：科技部农业成果转化项目(No.2006326018)资助;中央级公益性科研院所基本科研业务费专项(No.2007hzs1J003)

摘　　要：由橡胶树粉孢属(Oidium heveae B.A.Steinm)真菌侵染引起的橡胶树白粉病是橡胶树最严重的病害之一。从海南、广东、广西和云南等天然橡胶主产区共收集橡胶树白粉病菌样品20份,提取其基因组DNA进行rDNA-ITS区序列扩增,并对5.8SrDNA及其两侧ITS序列进行分析。通过序列结果分析发现,供试样品可分为2个群体。Powdery mildew of rubber tree （Hevea brasiliensis） caused by Oidium heveae Steinm is an important disease of rubber plantations worldwide. A total of 20 samples of rubber powder were collected from Hainan, Guangdong, Guangxi and Yunnan provinces in China, and their genomic DNAs were extracted. The ribosomal DNA internal transcribed spacer （ITS） region of the genomic DNAs was amplified and sequenced. Nucleotide sequence analysis showed that the Oidium heveae was divided into two groups.

关 键 词：巴西橡胶树 白粉病菌 内转录间隔区(ITS) PCR 

分 类 号：S763.741[农业科学—森林保护学]