天津市蓟县地区莱姆病螺旋体主要生物媒介的调查研究  

Investigation on Primary Vectors of Borrelia Burgdorferi in Ji County of Tianjin City

作　　者：侯学霞[1] 刘义[2] 郝琴[1] 陈继永[2] 耿震[1] 宋春英[2] 陈建[1] 丁健青[3] 于凤泰 贾艳合[2] 赵志武[2] 万康林[1] 

机构地区：[1]中国疾病预防控制中心传染病预防控制所,北京100050 [2]天津市蓟县卫生防病站 [3]天津市疾病预防控制中心

出　　处：《中国预防医学杂志》2008年第5期358-359,共2页Chinese Preventive Medicine

摘　　要：目的了解当地莱姆病的主要生物媒介—蜱的种类、带菌状况以及在传播莱姆病中的作用。方法采用布旗法及动物诱捕法在山林地区采集蜱,对蜱种进行鉴定。并进行病原分离培养和PCR检测。结果共收集到1226只蜱,经鉴定属于硬蜱科中2属2种:硬蜱属的全沟硬蜱和血蜱属的长角血蜱。其中以长角血蜱为优势种,构成比占92.58%(1135/1226);随机对300只长角血蜱进行针对莱姆病螺旋体的PCR检测,有14只阳性,阳性率为4.67%。并对其余926只蜱进行病原分离培养,未得到莱姆病螺旋体。结论血蜱可能是天津蓟县地区莱姆病传播的主要生物媒介。Objective To explore the species, bacteria carrying status and the role in causing Lyme disease of ticks. Methods ① Flagging and trapping methods were used to collect ticks in forest area and then the species of captured ticks were identified. ② BSK Ⅱ culture medium was used to isolate the pathogen. ③ PCR was used to detect the germ - carrying rate of ticks. Results A total of 1 226 ticks were collected, and then were indentified as Ixodidae （2 genera, 2 species）. Haemaphysalis longicornis was the dominant specie. The carrying -rate of Haemaphysalis longicornis in ticks was 4. 67% by PCR, but no pathogen was found by isolation or culture. Conclusion Haemaphysalis longicornis was an important vector in Ji County of Tianjin city.

关 键 词：莱姆病螺旋体 生物媒介 带菌状况 预防措施 传染病 

分 类 号：R181.3[医药卫生—流行病学]