血液细菌培养2254份与耐药性分析  

Analysis on Detected Bacteria and Drug Sensitivity from 2254 Blood Cultures

作　　者：刘筱玲[1] 杨长顺[1] 周细国[1] 周秀萍[2] 周旭[1] 

机构地区：[1]怀化市第一人民医院检验科,湖南怀化418000 [2]怀化医专检验系

出　　处：《怀化医专学报》2008年第1期15-17,共3页

摘　　要：目的探讨医院内血液感染的细菌种类及耐药性。方法用全自动血培养仪BACTEC9050进行培养，细菌鉴定采用ATB Expression系统，药敏试验采用K—B法。结果2254份血液细菌培养阳性率为10．20％，检出细菌23种共230株，其中革兰阳性细菌140株（60．87％），革兰阴性细菌76株（33．04％），真菌14株（6．09％）。革兰阳性细菌以表皮葡萄球菌（81／140）为主，革兰阴性茵中以大肠埃希茵（45／76）为主。表皮葡萄球菌对利福平、呋喃妥因、米诺环素、万古霉素和替考拉宁有较高敏感性。大肠埃希茵对派拉西林／他唑巴坦、头孢吡肟、阿米卡星、美罗培南和亚胺培南有较高敏感性。结论该院血液标本细菌检出率较高，了解血液感染细菌的分布特征和耐药性，对减少院内感染，指导临床合理使用抗生素有重要意义。Objective To investigate the kinds of bacteria and their drug resistance in blood cultures in hospitals. Methods Bacteria were detected with BACTEC - 9050 Autoanalyzer and ATB Expression. The K - B method was applied for the drug susceptibility test. Results The positive rate of blood cultures was 10.20 % and 230 bacterial strains were detected. There were 140 strains of Gram - positive bacteria （ 60.87 % ）, 76 strains of Gram - negative bacteria （ 33.04 % ） and 14 strain of fungus （6.09 % ） . The main bacteria in Gram - positive bacteria was Staphylococcus epidermidis and in Gram - negative bacteria was Escherichia coli. Staphylococcus epidermidis were highly sensitive to rifampicin, nitrofurantoin, minocycline, vancomycin and teicoplanin while Escherichia coli to piperacillin/tazobactam , cefepime, amikacin, imipenem and meropenem. Conclusion The isolated rate in blood culture is high. It was of great significance decreasing nosocomial infections and clinical chemotherapy by blood cultures.

关 键 词：血培养 细菌 耐药性 

分 类 号：R915[医药卫生—微生物与生化药学]