苯胺降解菌的分离和降解特性研究  

Study on isolation and degradation characters of a bacterial strain for aniline degradation

作　　者：刘宪军[1] 吕志堂[1] 张利平[1] 

机构地区：[1]河北大学生命科学学院,河北省微生物多样性研究与应用重点实验室,保定071002

出　　处：《环境工程学报》2008年第6期858-860,共3页Chinese Journal of Environmental Engineering

基　　金：保定市科学技术研究与发展计划资助(06F01);河北省科技资源数据库建设项目(07669045D-5)

摘　　要：通过驯化富集培养,从白洋淀底泥中分离筛选出数株能够有效降解苯胺的菌株,经过反复筛选,得到一株能够以苯胺为唯一碳源、高效降解苯胺的菌株BA-1-3。其利用苯胺的最适pH值为7.0,最适温度为30℃,在苯胺浓度为1 000 mg/L,180 r/min条件下振荡培养60 h,降解率达到80%以上。经鉴定,菌株BA-1-3属苍白杆菌属(O chrobactrumsp.)。Several aniline degrading strains were isolated from sluge samples collected from Baiyangdian by domestication enrichment culturing. Strain BA-1-3A, which can use aniline as sole carbon source and efficently degrade it, was obtained by repeatly screening. The optimum temperature and pH for aniline degradation were 30℃ and 7.0. After shaking for 60 h at 180 r/min at 1 000 mg/L of aniline and the above conditions, the aniline degradation rate was more than 80%. The strain BA-l-3 was idenfied as Ochrobactrum sp. preliminary.

关 键 词：苯胺 降解菌 分离筛选 降解特性 

分 类 号：Q939.9[生物学—微生物学]