清脂复肝颗粒防治大鼠酒精性脂肪肝的疗效及机制研究  

Study on effect and mechanism of Qingzhifugan granules on preventing alcoholic fatty liver in rats

作　　者：华海婴[1] 张转建[2] 付康[1] 杨波[1] 王曙炎[1] 戈士文[1] 叶启霞[1] 

机构地区：[1]郑州大学医药科学研究院,郑州450052 [2]郑州铁路职业技术学院,郑州450052

出　　处：《中华中医药杂志》2008年第6期487-489,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：河南省重大公益性科研计划项目资助课题(No.081100910600)

摘　　要：目的:探讨清脂复肝颗粒(qingzhifugan granules,QZFG)防治酒精性脂肪肝的作用及其可能的机制。方法:将QZFG分为6、3、1.5g/kg剂量组,进行预防给药,连续4周后,分别检测血清中天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)的含量;用免疫组化法检测肝组织中的半胱氨酸酶3(Caspase-3)表达水平;RT-PCR检测细胞色素P4502E1(CYP2E1)表达水平;并进行肝脏病理组织学检查。结果:QZFG各剂量组血清中AST、ALT的含量及Caspase-3、CYP2E1在肝组织中的表达水平明显低于模型组,且给药组之间差异明显,即表现出剂量相关性。病理组织学检查发现给药组肝脏脂肪变性程度明显轻于模型组。结论:QZFG可能通过降低肝脏中CYP2E1、Caspase-3水平发挥防治酒精性脂肪肝的作用。Objective： To explore the effect of Qingzhifugan Granules on alcoholic fatty liver（AFL） in rats and its possible mechanism.Methods： Preventive administration of Qingzhifufan Granule with high dose（6g/kg）,middle dose（3g/kg） and low dose（1.5g/kg） reapectively for 4 weeks,then,serum AST and ALT were tested.Expression of liver Caspase-3 was detected by immunohistochemistry,and CYP2E1 by semi-quantitative reverse transcription and polymerase chain reaction（RT-PCR）.Histopathological observation of liver was carried out too.Results： Compared with model group,the levels of serum AST and ALT as well as the expression of Caspase-3 and CYP2E1 in Qingzhifufan Granule treated groups decreased significantly.Significant difference between Qingzhifufan Granule treated groups were also observed,and have correlation with the dose administrated.Histopathological observation revealed the liver fatty degeneration of treated groups is much light than that of model groups.Conclusion： Qingzhifugan Granules has a protective effect on alcoholic fatty livers in a mechanism of decrease the expression of CYP2E1,Caspase-3 in liver.

关 键 词：酒精性脂肪肝 大鼠 中医药疗法 清脂复肝颗粒 

分 类 号：R285.5[医药卫生—中药学]