蜡样芽胞杆菌B905 hblA基因的初步分析  

Detection of Hemolysin BL and Analyses of hblA Gene in Bacillus cereus 905

作　　者：王利国[1] 王琦[1] 王勇军[1] 梅汝鸿[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100094

出　　处：《微生物学杂志》2008年第2期6-10,共5页Journal of Microbiology

基　　金：国家自然科学基金项目(30640044)

摘　　要：采用血平板培养的方法对蜡样芽胞杆菌905菌株溶血素BL检测,并通过PCR方法克隆其基因,结果表明该菌株产生溶血环且含有hblA、hblC、hblD溶血素BL全部基因;采用同源重组法构建了该菌株hblA基因缺失突变体,结果该菌株的溶血活性并未发生改变,可能是由于该菌株溶血素基因的结构与Handelsman构建所用的菌株Bacillus cereus UW85有一定的差异,或者是由于突变位点在阅读框内后端,未能真正破坏其表达。还需要进一步对其进行研究。Adopting sheep blood agar plate culture hemolysin BL in Bacillus cereus 905 was detected by PCR method. The results indicated that the strain produced hemolytic ring and contains all hemolysin BL genes hblA, hblC, and hblD. hblA gene mutant was constructed adopted homologous recombination method, however, the hemolytic activity remained unchanged, probably because there were a certain diversity between the structure of hemolytic genes of the strain and Bacillus cereus UW85 used and constructed by Handelsman, or because the mutation site of the strain was at back end of the reading frame that their expression was not really destroyed. These will need further study.

关 键 词：蜡样芽胞杆菌 溶血素BL 检测 突变体 

分 类 号：Q939.124[生物学—微生物学]